The seminal fluid proteome of the honeybee Apis mellifera
The seminal fluid proteome of the honeybee Apis mellifera
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DOI：
10.1002/pmic.200800708
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发表时间：
2009-04-01
期刊：
影响因子：
3.4
通讯作者：
Millar, A. Harvey
中科院分区：
文献类型：
--
作者：
Baer, Boris;Heazlewood, Joshua L.;Millar, A. Harvey
射精包含精子，但也有精液，这是越来越多地认识到生殖成功的核心重要性。然而，精液的详细生化成分和生理学理解仍然是难以捉摸的。我们已经使用MS来确定57个最丰富的蛋白质内的蜜蜂Apis mellifiera的精液。它们的氨基酸序列揭示了酶、调节剂和结构蛋白的不同功能类别的存在。一些已知或预测的作用，在维持精子活力，保护精子免受微生物感染或与女性的生理相互作用。在57个精子中检测到一系列推定的糖蛋白或糖基化酶，随后的糖酵解荧光染色显示精液中有几种糖蛋白，而精子样品中没有检测到糖蛋白。许多在精液中积累的丰富蛋白质并不包含可预测的细胞分泌标签。蜜蜂精液蛋白质与果蝇精液蛋白质（包括分泌的附属腺蛋白，称为ACP），并与人类精液蛋白质组的比较显示，蜜蜂蛋白质组包含一系列新鉴定的精液蛋白质，我们注意到蜜蜂蛋白质组与当前人类精液蛋白质组比已知的果蝇精液蛋白质组更相似。因此，蜜蜂精液蛋白质组代表了一个重要的除了现有的数据比较研究的昆虫精液蛋白质组。
Ejaculates contain sperm but also seminal fluid, which is increasingly recognized to be of central importance for reproductive success. However, a detailed biochemical composition and physiological understanding of seminal fluid is still elusive. We have used MS to identify the 57 most abundant proteins within the ejaculated seminal fluid of the honeybee Apis mellifiera. Their amino acid sequences revealed the presence of diverse functional categories of enzymes, regulators and structural proteins. A number have known or predicted roles in maintaining sperm viability, protecting sperm from microbial infections or interacting with the physiology of the female. A range of putative glycoproteins or glycosylation enzymes were detected among the 57, subsequent fluorescent staining of glycolysation revealed several prominant glycoproteins in seminal fluid, while no glycoproteins were detected in sperm samples. Many of the abundant proteins that accumulate in the seminal fluid did not contain predictable tags for secretion for the cell. Comparison of the honeybee seminal fluid proteins with Drosophila seminal fluid proteins (including secreted accessory gland proteins known as ACPs), and with the human seminal fluid proteome revealed the bee protein set contains a range of newly identified seminal fluid proteins and we noted more similarity of the bee protein set with the current human seminal fluid protein set than with the known Drosophila seminal fluid proteins. The honeybee seminal fluid proteome thus represents an important addition to available data for comparative studies of seminal fluid proteomes in insects.