Planar spindle orientation and asymmetric cytokinesis in the mouse small intestine
Planar spindle orientation and asymmetric cytokinesis in the mouse small intestine
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DOI：
10.1369/jhc.7a7234.2007
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发表时间：
2007-11-01
影响因子：
3.2
通讯作者：
Tirnauer, Jennifer S.
中科院分区：
文献类型：
--
作者：
Fleming, Elizabeth S.;Zajac, Mark;Tirnauer, Jennifer S.
上皮细胞极性的一个主要特征是有丝分裂纺锤体在细胞内的位置受到调节。在对称扩张的组织中，细胞中的纺锤体可以与组织平面平行排列。在哺乳动物组织中直接测量这种排列是困难的。在这里，我们利用微管免疫荧光和三维共聚焦成像分析了完整小鼠肠上皮中纺锤波的位置。在肠隐窝增殖区发现有丝分裂细胞。主轴角相对于顶细胞表面是确定或直接测量从共聚焦图像或与计算算法。在中期和后期细胞中，纺锤体的角度与顶面平行的平均角度在10度以内，与强大的平面纺锤体定位一致，而在前期细胞中，纺锤体的角度变化要大得多。有趣的是，细胞动力学沟似乎从基底细胞表面向顶表面延伸。这种类型的图像分析可能有助于研究组织重塑和肿瘤形成过程中纺锤体位置的调节。
A major feature of epithelial cell polarity is regulated positioning of the mitotic spindle within the cell. Spindles in cells of symmetrically expanding tissues are predicted to align parallel to the tissue plane. Direct measurement of this alignment has been difficult in mammalian tissues. Here, we analyzed the position of spindles in intact mouse intestinal epithelium using microtubule immunofluorescence and three-dimensional confocal imaging. Mitotic cells were identified in the proliferative zone of intestinal crypts. Spindle angle relative to the apical cell surface was determined either by direct measurement from confocal images or with a computational algorithm. Angles averaged within 10 degrees of parallel to the apical surface in metaphase and anaphase cells, consistent with robust planar spindle positioning, whereas spindles in prometaphase cells showed much greater angle variability. Interestingly, cytokinetic furrows appeared to extend from the basal cell surface toward the apical surface. This type of image analysis may be useful for studying the regulation of spindle position during tissue remodeling and tumor formation.