Use of Bacteriophage-resistant Mutants to Study the Nature of the Bacteriophage Receptor Site of Staphylococcus aureus
Use of Bacteriophage-resistant Mutants to Study the Nature of the Bacteriophage Receptor Site of Staphylococcus aureus
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利用噬菌体抗性突变体研究金黄色葡萄球菌噬菌体受体位点的性质
DOI：
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发表时间：
1969
影响因子：
3.2
通讯作者：
A. Chatterjee
中科院分区：
文献类型：
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作者：
A. Chatterjee
用N-甲基-N′-硝基-N-亚硝基胍对金黄色葡萄球菌H株进行诱变，筛选出耐噬菌体的菌株。从这些耐药菌株中分离的约一半的细胞壁不能灭活β-葡糖胺，并且显示其细胞壁磷壁酸中缺乏N-乙酰基-D-葡糖胺（GlcNAc）。除了缺乏GlcNAc，这些突变株中有两个缺乏细胞壁磷和酯连接的d-丙氨酸。还发现这两种菌株对噬菌体K和从亲本噬菌体分离的宿主范围突变体都具有抗性。这两种酶能裂解其他磷壁酸中缺乏GlcNAc的抗噬菌体突变体。来自剩余的噬菌体抗性突变株的细胞壁没有降解，并且发现具有正常的细胞壁磷壁酸。虽然磷壁酸中的GlcNAc是噬菌体灭活所必需的，但从S.金黄色葡萄球菌，其细胞壁磷壁酸中仅具有α-或β-连接的GlcNAc。
Bacteriophage-resistant strains of Staphylococcus aureus H were isolated after mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. Cell walls isolated from about half of these resistant strains were incapable of inactivating phages and were shown to lack N-acetyl-d-glucosamine (GlcNAc) in their cell wall teichoic acid. Apart from the lack of GlcNAc, two of these mutant strains were deficient in cell wall phosphorus and ester-linked d-alanine. These two strains were also found to be resistant to both phage K and a host-range mutant isolated from the parent phage. These two phages could lyse the other phage-resistant mutants which lacked GlcNAc in their teichoic acid. Cell walls from the remaining phage-resistant mutant strains did inactivate phages and were found to have normal cell wall teichoic acid. Although GlcNAc in teichoic acid was required for phage inactivation, no difference in phage inactivation ability was detected with cell walls isolated from strains of S. aureus having exclusively α- or exclusively β-linked GlcNAc in their cell wall teichoic acid.