Effects of Chronic Bhang (Cannabis) Administration on the Reproductive System of Male Mice
Effects of Chronic Bhang (Cannabis) Administration on the Reproductive System of Male Mice
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DOI：
10.1002/bdrb.20295
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发表时间：
2011-06-01
影响因子：
--
通讯作者：
Krishna, Amitabh
中科院分区：
文献类型：
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作者：
Banerjee, Arnab;Singh, Ajit;Krishna, Amitabh
背景：本研究的目的是研究长期摄取从大麻中制备的大麻对成年雄性 Parkes 品系 (P) 小鼠的雄性生殖生理学的影响。还尝试调查睾丸中大麻素 1 (CB1) 和大麻素 2 (CB2) 受体以及脂肪酸酰胺水解酶 (FAAH) 的存在，并评估小鼠长期摄入大麻导致的任何变化。方法和结果：成年雄性小鼠连续36天口服bhang（3或6毫克/公斤体重/天）。长期摄入大麻会导致睾丸发生退化性变化，并抑制精子数量、活力和活力。由于睾丸 3β HSD 酶活性下降，摄入苯丙胺还导致循环睾酮水平显着下降。免疫组织化学研究表明小鼠睾丸中存在 CB1、CB2 和 FAAH。本研究还表明，暴露于大麻的小鼠睾丸中的 CB1 和 CB2 受体以及 FAAH 蛋白水平存在显着变化。这些抑制作用可能是由于 bhang 对垂体促性腺激素释放激素 (GnRH) I 受体蛋白表达的抑制作用。体外用 bhang 处理睾丸显着降低了睾丸黄体生成素受体 (LHR) 和 FAAH 的表达，表明 bhang 对睾丸活性有直接作用。结论：这项研究的结果表明，大麻可能通过改变睾丸内源性大麻素系统来损害雄性小鼠的生育能力，并且人类长期接触大麻可能会改变雄性的生育能力。出生缺陷研究（B 部分）92:195-205，2011。(C)2011 Wiley-Liss, Inc.
BACKGROUND: The purpose of this study was to investigate the effect of chronic uptake of bhang, prepared from the Cannabis sativa, on male reproductive physiology in adult male Parkes strain (P) mice. An attempt was also made to investigate the presence of cannabinoid 1 (CB1) and cannabinoid 2 (CB2) receptors, and fatty acid amide hydrolase (FAAH) in the testis and to evaluate any changes in it resulting from chronic intake of bhang in mice. METHODS AND RESULTS: Adult male mice were given bhang (3 or 6 mg/kg body weight/day) orally for 36 consecutive days. Chronic intake of bhang caused regressive changes in the testes and suppressed sperm count, viability and motility. Bhang intake also caused significant decline in circulating testosterone level due to decline in testicular 3 beta HSD enzyme activity. An immunohistochemical study demonstrated the presence of CB1, CB2 and FAAH in the testis of mice. The present study also showed significant variation in the CB1 and CB2 receptors and FAAH protein levels in testes of mice exposed to bhang. These suppressive effects may be due to inhibitory effect of bhang on pituitary expression of gonadotrophin releasing hormone (GnRH) I receptor protein. Treatment of testes with bhang in vitro significantly decreased testicular luteinizing hormone receptor (LHR) and FAAH expression suggesting direct action of bhang on testicular activity. CONCLUSIONS: The findings of this study thus suggest that bhang may impair fertility in male mice through alteration in the testicular endocannabinoid system and that chronic bhang exposure in humans would be predicted to alter male fertility. Birth Defects Res (Part B) 92:195-205, 2011. (C) 2011 Wiley-Liss, Inc.