Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications
Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications
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DOI：
10.1016/j.ymeth.2014.10.009
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发表时间：
2015-04-01
期刊：
影响因子：
4.8
通讯作者：
Bonkat, G.
中科院分区：
文献类型：
--
作者：
Braissant, O.;Bachmann, A.;Bonkat, G.
等温微量热法测量物理或化学过程释放或消耗的热量。代谢活动释放的热量是可以测量的。然而，新用户可能难以解释量热信号。本文比较了微量热法与其他技术，并回顾了其在微生物活性重要的不同领域的应用。我们还描述了不同的方法来分析数据，并将其转化为有意义的（微）生物等效物。本文旨在为非专业读者提供了解等温微量热法如何用于微生物学应用的工具。(C)2014 Elsevier Inc. All rights reserved.
Isothermal microcalorimetry measures the heat released or consumed by physical or chemical processes. Metabolic activity releases heat that can be measured. However the calorimetric signal can be difficult for new users to interpret. This paper compares microcalorimetry to other techniques and reviews its application to different fields where microbiological activity is important. We also describe different ways to analyze the data and translate it into meaningful (micro)biological equivalents. This paper aims at providing non-specialist reader the tools to understand how isothermal microcalorimetry can be used for microbiological applications. (C) 2014 Elsevier Inc. All rights reserved.