LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea, and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia
LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea, and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia
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DOI：
10.3390/md8041049
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发表时间：
2010-04-01
期刊：
影响因子：
5.4
通讯作者：
Yotsu-Yamashita, Mari
中科院分区：
文献类型：
--
作者：
Jang, Jun-Ho;Lee, Jong-Soo;Yotsu-Yamashita, Mari
对韩国南部沿海河豚鱼（Fugu niphobles）的3只雌性和3只雄性标本的组织和全身中的河豚毒素（TTX）及其脱氧类似物5-deoxyTTX、11-deoxyTTX、6，11-dideoxyTTX和5，6，11-trideoxyTTX进行了定量分析。采用LC/MS单离子模式（SIM）对东南亚的黑绿河豚（Tetroodonnigroviridis）（12个标本）和双斑河豚（Tetroodonbiocellatus）（3个标本）进行分析。这四种脱氧类似物在F.三种河豚鱼中均检测到TTX和5，6，11-三脱氧TTX，与日本东方鲀相似，为主要的TTX类似物。而6，11-双脱氧TTX也被发现是几乎所有朝鲜人组织的主要类似物。nifophobles，这种类似物是次要的，在两个Tetraodon物种和日本F。pardalis。在F.在nifophobles中，TTX的浓度在卵巢（雌性）和皮肤（雌性和雄性）中最高。
Tetrodotoxin (TTX) and its deoxy analogs, 5-deoxyTTX, 11-deoxyTTX, 6,11-dideoxyTTX, and 5,6,11-trideoxyTTX, were quantified in the tissues of three female and three male specimens of the marine puffer fish, Fugu niphobles, from the southern coast of Korea, and in the whole body of the brackishwater puffer fishes, Tetraodon nigroviridis (12 specimens) and Tetrodon biocellatus (three specimens) from Southeast Asia using LC/MS in single ion mode (SIM). Identification of these four deoxy analogs in the ovarian tissue of F. niphobles were further confirmed by LC/MS/MS. TTX and 5,6,11-trideoxyTTX were detected in all three puffer fish species as the major TTX analogs, similar to Japanese Fugu pardalis. While 6,11-dideoxyTTX was also found to be a major analog in almost all tissues of Korean F. niphobles, this analog was minor in the two Tetraodon species and Japanese F. pardalis. Among the tissues of F. niphobles, the concentrations of TTXs were highest in the ovaries (female) and skin (female and male).