Facile colorimetric detection of 6-benzylaminopurine based on p-aminobenzenethiol functionalized silver nanoparticles
Facile colorimetric detection of 6-benzylaminopurine based on p-aminobenzenethiol functionalized silver nanoparticles
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DOI：
10.1039/c6ra21838e
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发表时间：
2016-11
期刊：
影响因子：
3.9
通讯作者：
Shuang Ma;Jiang He;Mingzhen Guo;Xiaohan Sun;Mingda Zheng
中科院分区：
文献类型：
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作者：
Shuang Ma;Jiang He;Mingzhen Guo;Xiaohan Sun;Mingda Zheng
利用新型探针对氨基苯硫醇功能化纳米银（ABT-AgNPs），建立了一种在复杂环境中检测6-苄基氨基嘌呤（6-BA）的简单比色法。当ABT-AgNPs与6-BA结合时，基于强烈的静电效应、氢键和π -π堆叠相互作用，形成一个巨大的共轭网络，可见颜色由淡黄色变为红橙色。通过密度泛函理论（DFT）验证了6-BA与ABT之间的相互作用。系统地研究了实验条件对分析性能的影响。在最佳条件下，吸光度（A510 nm/ a397nm）与6-BA浓度的对数在1 μM ~ 20 μM范围内呈线性关系，检出限为0.1 μM。普通无机离子和其他农药对其无明显干扰。6-BA在复杂基质样品中也能成功测定。水和蔬菜样品的回收率在~ 81.3 ~ 101.2%之间。
A simple colorimetric assay has been developed to detect 6-benzylaminopurine (6-BA) in a complex environment by using the novel probe p-aminobenzenethiol functionalized silver nanoparticles (ABT-AgNPs). When ABT-AgNPs bind with 6-BA, a huge conjugate network can form based on intense electrostatic effects, hydrogen bonding and π–π stacking interactions, resulting in a visible colour change from faint yellow to reddish orange. The interactions between the 6-BA and ABT also were validated by the density functional theory (DFT) approach. The influence of experimental conditions on the analytical performance was investigated systematically. Under optimal conditions, the absorbance ratio (A510 nm/A397 nm) is linearly related to the logarithm of 6-BA concentration from 1 μM to 20 μM, with a detection limit of 0.1 μM. Furthermore, no obvious interference was observed from common inorganic ions and other pesticides. 6-BA was also successfully determined in samples with complicated matrices. The recoveries obtained from water and vegetable samples were in the range ∼81.3–101.2%.