HELIX FORMATION BETWEEN POLYRIBONUCLEOTIDES AND PURINES PURINE NUCLEOSIDES AND NUCLEOTIDES .2.
HELIX FORMATION BETWEEN POLYRIBONUCLEOTIDES AND PURINES PURINE NUCLEOSIDES AND NUCLEOTIDES .2.
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DOI：
10.1016/s0022-2836(66)80183-3
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发表时间：
1966-01-01
影响因子：
5.6
通讯作者：
MILES, HT
中科院分区：
文献类型：
--
作者：
HOWARD, FB;FRAZIER, J;MILES, HT
许多单体核酸组分与多核苷酸反应形成具有螺旋结构的明确的复合物，类似于在相应的高聚物对之间形成的复合物。单体可以是嘌呤或嘌呤核苷或核苷酸，但尚未发现嘧啶衍生物形成这样的配合物。这一现象已通过红外光谱和旋光法以及利用一种已知对二级结构敏感的酶进行了研究。观察到相互作用的碱基配对特异性，并且组分以确定的化学计量比反应。某些配合物的稳定性取决于反应物的浓度、盐的浓度和温度。
A number of monomeric nucleic acid components react with polynucleotides to form well-defined complexes having helical structures similar to those formed between corresponding pairs of high polymers. The monomer can be a purine or a purine nucleoside or nucleotide, but no pyrimidine derivative has yet been found to form such complexes. The phenomenon has been investigated by infrared spectroscopy and optical rotation and by use of an enzyme of known sensitivity to secondary structure. Base-pairing specificity of interaction is observed, and the components react in definite stoichiometric ratios. The dependence of the stabilities of some of the complexes upon concentration of reactants, concentration of salt, and upon temperature has been determined.