Molecular identification of IgE-dependent histamine-releasing factor as a B cell growth factor
Molecular identification of IgE-dependent histamine-releasing factor as a B cell growth factor
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DOI：
10.4049/jimmunol.166.11.6545
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发表时间：
2001-06-01
影响因子：
4.4
通讯作者：
Choi, I
中科院分区：
文献类型：
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作者：
Kang, HS;Lee, MJ;Choi, I
小鼠红白血病细胞LK1的培养上清液具有B细胞刺激活性。纯化和NH2末端序列分析表明，其中一个候选基因是小鼠IgE依赖的组胺释放因子(IgE-HRF)，它可以从嗜碱性粒细胞中诱导组胺。从大肠杆菌或293转化的胚胎肾细胞中获得重组IgE-HRF(RHRF)，检测其B细胞刺激活性。两种rHRF均以剂量依赖方式刺激B细胞增殖。然而，煮沸或抗HRF单抗可取消rHRF对B细胞的刺激作用。重组人绒毛膜促性腺激素与IL-2、IL-4、IL-5具有很强的协同作用，在抗CD40抗体存在的情况下活性最高。重组人绒毛膜促性腺激素能增加B细胞MHCⅡ类分子的表达。它还增加了B细胞产生Ig的能力。多粘菌素B是一种脂多糖的中和肽抗生素，但不降低rHRF的活性。此外，用PE标记的rHRF进行流式细胞仪分析表明，HRF与B细胞结合。重组HRF上调B细胞IL-1和IL-6的表达。体内注射rHRF或rHRF的cDNA可增加总免疫球蛋白的合成和抗原特异性免疫球蛋白的合成。综上所述，这些结果表明，HRF刺激B细胞的激活和功能。
The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH2-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 Ab. Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.