ANTICRYPTOCOCCAL RESISTANCE IN THE MOUSE-BRAIN - BENEFICIAL-EFFECTS OF LOCAL-ADMINISTRATION OF HEAT-INACTIVATED YEAST-CELLS
ANTICRYPTOCOCCAL RESISTANCE IN THE MOUSE-BRAIN - BENEFICIAL-EFFECTS OF LOCAL-ADMINISTRATION OF HEAT-INACTIVATED YEAST-CELLS
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DOI：
10.1128/iai.62.8.3189-3196.1994
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发表时间：
1994-08-01
影响因子：
3.1
通讯作者：
BISTONI, F
中科院分区：
文献类型：
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作者：
BLASI, E;MAZZOLLA, R;BISTONI, F
使用小鼠模型，我们先前已经证明，大脑对局部感染机会性真菌的抵抗力受到宿主粒单核细胞隔室的操纵的影响。在这里，我们证明了脑内注射热灭活的新生隐球菌(H-CN)酵母细胞可以持续提高小鼠的存活率，以应对随后致死剂量的新生隐球菌的局部攻击。这种现象在两次H-CN治疗时更加明显，与(I)大量局部炎症反应，(Ii)减少脑内真菌的生长，以及(Iii)诱导迟发性超敏反应有关。此外，H-CN处理提供了针对本地异源挑战的保护。我们的数据提供了脑内注射H-CN导致建立非特异性和特异性免疫反应的初步证据；诱导机制和对宿主抗菌素耐药性的相对贡献仍有待阐明。
Using a murine model, we have previously shown that brain resistance to local infection with opportunistic fungi is affected by manipulation of the host myelomonocytic compartment. Here, we demonstrate that intracerebral administration of heat-inactivated Cryptococcus neoformans (H-CN) yeast cells results in a consistent enhancement of mouse survival to subsequent local challenge with lethal doses of C. neoformans. The phenomenon, more pronounced upon double H-CN treatment, is associated with (i) massive local inflammatory response, (ii) reduced growth of the fungus within the brain, and (iii) induction of delayed-type hypersensitivity. Moreover, H-CN treatment confers protection against local heterologous challenges. Our data provide initial evidence that intracerebral administration of H-CN results in the establishment of aspecific and specific immune responses; the mechanisms of elicitation and relative contributions to host antimicrobial resistance remain to be elucidated.