CORRELATION BETWEEN CELL ENLARGEMENT AND NUCLEIC ACID AND PROTEIN CONTENT OF HELA CELLS IN UNBALANCED GROWTH PRODUCES BY INHIBITORS OF DNA SYNTHESIS
CORRELATION BETWEEN CELL ENLARGEMENT AND NUCLEIC ACID AND PROTEIN CONTENT OF HELA CELLS IN UNBALANCED GROWTH PRODUCES BY INHIBITORS OF DNA SYNTHESIS
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DOI：
10.1002/jcp.1040690309
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发表时间：
1967-01-01
影响因子：
5.6
通讯作者：
STUDZINSKI, GP
中科院分区：
文献类型：
--
作者：
COHEN, LS;STUDZINSKI, GP
用以下DNA合成抑制剂丝裂霉素C、氮芥、氟脱氧尿苷、羟基脲、阿拉伯呋喃糖基胞嘧啶和高浓度胸苷处理单层培养物中的HeLa细胞。在大多数情况下，所用的每种抑制剂的浓度仅足以阻止细胞增殖，并且在RNA和蛋白质的合成仅被部分抑制而DNA合成停止的意义上，所有抑制剂都产生不平衡的生长。这导致在48小时内RNA和蛋白质含量/细胞增加约100%，并且由于在此期间细胞体积也增加100%，因此RNA和蛋白质浓度/单位细胞体积保持恒定。细胞蛋白质含量可作为DNA合成抑制剂处理的HeLa细胞中细胞大小变化的准确指标。
HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis mitomycin C; nitrogen mustard; fluorodeoxyuridine; hydroxyurea; arabinofuranosylcytosine and high concentrations of thymidine. The concentration of each inhibitor used was, in most cases, just sufficient to arrest cell multiplication and all produced unbalanced growth in the sense that the synthesis of RNA and protein were only partially inhibited while DNA synthesis stopped. This resulted in approximately 100% increases in RNA and protein content/cell in 48 hr. and, since cell volume also increased by 100% during this time, the concentration of RNA and protein/unit cell volume remained constant. Cell protein content may be used as an accurate index of variation in cell size in HeLa cells treated with inhibitors of DNA synthesis.