Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status.
Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status.
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DOI：
10.1186/s12977-016-0263-z
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发表时间：
2016-04-27
期刊：
影响因子：
3.3
通讯作者：
Saito M
中科院分区：
文献类型：
--
作者：
Shiohama Y;Naito T;Matsuzaki T;Tanaka R;Tomoyose T;Takashima H;Fukushima T;Tanaka Y;Saito M
人类T细胞白血病病毒1型（HTLV-1）碱性亮氨酸拉链因子（HBZ）被认为在成人T细胞白血病（ATL）和HTLV-1相关脊髓病/热带痉挛性麻痹（HAM/TSP）的发展中起重要作用。然而，在不同临床状态的HTLV-1感染个体中，对HBZ的mRNA和蛋白表达、对HBZ的体液免疫反应性和HTLV-1前病毒载量（PVL）进行综合分析，此前尚未见报道。在这项研究中，我们使用新型单克隆抗体为基础的内部酶联免疫吸附测定系统，报告了不同临床状态htlv -1感染个体临床样品中HBZ蛋白及其血浆抗体的绝对定量。比较HBZ mRNA水平和PVL水平。结果显示，无症状携带者（ACs）的10.4%（5/48）、HAM/TSP患者的10.8%（13/120）、ATL患者的16.7%（7/42）血浆中检测到抗hbz抗体。5例急性ATL患者中有3例检测到HBZ蛋白，但在HAM/TSP（0/10）或ACs（0/4）患者中未检测到HBZ蛋白。因此，对HBZ的抗体反应与PVL或HBZ的表达（在mRNA和蛋白水平上）或感染的临床状态无关。目前的结果强调HBZ在自然HTLV-1感染中极低的表达和免疫原性。然而，PBMCs中HBZ蛋白的低但明显的表达可能与htlv -1感染细胞的存活和ATL的发生有关。本文的在线版本（doi:10.1186/s12977-016-0263-z）包含补充材料，可供授权用户使用。
Human T cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor (HBZ), which is encoded by a minus strand mRNA, is thought to play important roles in the development of adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). However, a comprehensive analysis of HBZ, including mRNA and protein expression, humoral immunoreactivity against HBZ, and HTLV-1 proviral load (PVL), in HTLV-1-infected individuals with different clinical status has not been reported previously. In this study, using novel monoclonal antibody-based in-house enzyme-linked immunosorbent assay systems, we report the absolute quantification of HBZ protein and its plasma antibody in clinical samples from HTLV-1-infected individuals with different clinical status. The data were compared to both HBZ mRNA levels and PVL. The results showed that plasma anti-HBZ antibody was detectable only in 10.4 % (5/48) of asymptomatic carriers (ACs), 10.8 % (13/120) of HAM/TSP patients, and 16.7 % (7/42) of ATL patients. HBZ protein was detected in three out of five patients with acute ATL, but was not detected in patients with HAM/TSP (0/10) or ACs (0/4). Thus, an antibody response to HBZ was not associated with the PVL or the expression of HBZ (both at the mRNA and protein levels) or the clinical status of the infection. The present results emphasize the extremely low expression and immunogenicity of HBZ in natural HTLV-1 infection. However, there is a possibility that the low but distinct expression of HBZ protein in PBMCs is associated with the survival of HTLV-1-infected cells and the development of ATL. The online version of this article (doi:10.1186/s12977-016-0263-z) contains supplementary material, which is available to authorized users.