Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
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DOI：
10.1111/j.1365-2761.2011.01291.x
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发表时间：
2011-10-01
影响因子：
2.5
通讯作者：
Buchmann, K.
中科院分区：
文献类型：
--
作者：
Jorgensen, L. von Gersdorff;Heinecke, R. D.;Buchmann, K.
淡水鱼能够在非致死性接触后对寄生虫Ichthyophthirius multifiliis（Ich）产生保护性免疫反应。参与免疫的因素包括细胞和体液因素，但抗体已被认为在保护中发挥突出作用。然而，宿主抗体尚未被证明与寄生虫原位结合。通过使用免疫组织化学技术，本研究表明，IgT和IgM结合到表面结构，包括纤毛，在早期喂养阶段的寄生虫在鳃的免疫虹鳟鱼，虹鳟鱼，入侵后不久（2小时）。没有结合的IgT和没有或只有一个弱的IgM结合上观察到类似的暴露，但天真的虹鳟鱼鳃中的寄生虫。这项研究表明，抗体在免疫鱼对Ich的保护中起着重要的作用，尽管额外的体液和细胞因子可能有助于这种反应。
Freshwater fish are able to mount a protective immune response against the parasite Ichthyophthirius multifiliis (Ich) following a non-lethal exposure. Factors involved in immunity comprise cellular and humoral factors, but antibodies have been suggested to play a prominent role in protection. However, host antibodies have not yet been demonstrated to bind to the parasite in situ. By the use of immunohistochemical techniques, this study demonstrated that IgT and IgM bind to surface structures, including cilia, on the early feeding stage of the parasite in the gills of immune rainbow trout, Oncorhynchus mykiss, shortly (2 h) after invasion. No binding of IgT and no or only a weak binding of IgM was observed on the parasites in the gills of similarly exposed but naive rainbow trout. This study indicates that antibodies play an important part in the protection of immune fish against Ich although additional humoral and cellular factors may contribute to this reaction.