Size discrimination in rat and mouse gastric emptying
Size discrimination in rat and mouse gastric emptying
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DOI：
10.1002/bdd.1828
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发表时间：
2013-03-01
影响因子：
2.1
通讯作者：
McConville, Jason T.
中科院分区：
文献类型：
--
作者：
Jang, Shih-Fan;Goins, Betha.;McConville, Jason T.
目的研究放射性标记不溶性聚甲基丙烯酸甲酯（PMMA）微胶囊/微珠与小鼠胃排空的关系。方法将PMMA微胶囊（50500 μ m）和载99m二乙烯三胺五乙酸（99mTc-DTPA）的微珠（0.53mm）灌胃给ICR小鼠或SD大鼠。伽玛扫描最初在给药后进行，然后每隔一小时至4小时进行一次。结果扫描结果显示，两种啮齿类动物中最小的PMMA微胶囊（50100 μ m）或微珠（0.51mm）在胃中受阻，排空速度比大颗粒慢。在小鼠中，直径在100和300 μ m之间的微胶囊对胃排空无显著差异（p=0.25），超过300 μ m的颗粒不能给药。在大鼠中，含有0.53mm微球的胶囊被粘在食道上（长达1小时），这是这种大小的微球给药的局限性，因为它们不能悬浮在液体介质中用于口服灌胃。直径为23mm的珠粒在胃中停留长达4小时。结论由于现有给药方法的限制，无法确定ICR小鼠的截止排空大小。SD大鼠的截空尺寸在1.5 ~ 2mm之间。因此，粒径大于2mm的颗粒不宜用于SD大鼠完整颗粒胃排空研究，因为颗粒在胃内排空迟缓。版权所有：John Wiley & Sons， Ltd。
Objectives To investigate the relationship between particle size and gastric emptying in rodents using radiolabeled insoluble polymethyl methacrylate (PMMA) microcapsules/beads. Methods PMMA microcapsules (50500 mu m) and beads (0.53mm) loaded with technetium-99m diethylenetriamine pentaacetic acid (99mTc-DTPA) were administered to ICR mice or Sprague Dawley (SD) rats by oral gavage. Gamma scintiscans were acquired initially following administration and then at hourly intervals to 4hours. Results Scintiscans revealed that the smallest PMMA microcapsules (50100 mu m) or beads (0.51mm) were impeded in the stomach and emptied slower than large particles in both rodent species. In mice, no significant difference in gastric emptying was found with microcapsules between 100 and 300 mu m in diameter (p=0.25) and particles more than 300 mu m could not be administered. In rats, capsules containing 0.53mm beads were stuck to the esophagus (up to 1hour), this was a limitation of dosing beads of this size because they cannot be suspended in a liquid media for oral gavage purposes. Beads with diameters of 23mm stayed in the stomach for up to 4hours. Conclusions The cut-off emptying size in ICR mice could not be determined, due to the limitation of current available dosing methods. The cut-off emptying size in SD rats was between 1.5 and 2mm. Therefore, particles with a diameter greater than 2mm should not be used for gastric emptying studies of intact particles in SD rats, as their emptying is retarded in the stomach. Copyright (c) 2012 John Wiley & Sons, Ltd.