Cloning and characterization of a novel lipase from Stenotrophomonas maltophilia GS11: The first member of a new bacterial lipase family XVI.
Cloning and characterization of a novel lipase from Stenotrophomonas maltophilia GS11: The first member of a new bacterial lipase family XVI.
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DOI：
10.1016/j.jbiotec.2016.04.034
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发表时间：
2016-06
影响因子：
4.1
通讯作者：
Mu Li;Lirong Yang;Gang Xu;Jianping Wu
中科院分区：
文献类型：
--
作者：
Mu Li;Lirong Yang;Gang Xu;Jianping Wu
细菌脂肪酶是一类重要的酶，在有机合成中具有巨大的潜力，鉴定和开发新的细菌脂肪酶具有很大的兴趣。在以往的研究中，证实了窄养单胞菌属的菌株是脂肪酶的潜在来源，但关于窄养单胞菌属脂肪酶的遗传信息很少。我们已经克隆并鉴定了一种新的脂肪酶（LipSM54），这是第一个从窄养单胞菌中描述的脂肪酶。酶学研究表明，LipSM54是一种冷活性、耐溶剂、碱性脂肪酶。利用生物信息学工具，LipSM54被发现仅与来自不同细菌起源的几种假定的脂肪酶有关，其中没有一个可以分配给任何先前描述的细菌脂肪酶家族。LipSM54和这些相关的假定脂肪酶在催化残基周围共享四个保守基序。这些基序清楚地将它们与已知的细菌脂肪酶家族区分开来。因此，LipSM54是新型细菌脂肪酶家族的第一个特征成员。
Bacterial lipases are an important group of enzymes that offer enormous potential in organic synthesis, and there is considerable interest in identifying and developing novel bacterial lipases. In previous studies, strains of the genusStenotrophomonaswere proved to be potential source of lipases, but there is little genetic information describing lipase from the genusStenotrophomonas. We have cloned and characterized a novel lipase (LipSM54), the first lipase described from the genusStenotrophomonas. Enzymatic study showed that LipSM54 was a cold-active, solvent-tolerant and alkaline lipase. Using bioinformatics tools, LipSM54 was found to be related only to several putative lipases from different bacterial origins, none of which could be assigned to any previously described bacterial lipase family. LipSM54 and these related putative lipases share four conserved motifs around the catalytic residues. These motifs clearly distinguish them from the known bacterial lipase families. Consequently, LipSM54 is the first characterized member of the novel bacterial lipase family.