Large-scale biochemical profiling of the Candida albicans biofilm matrix: new compositional, structural, and functional insights.
Large-scale biochemical profiling of the Candida albicans biofilm matrix: new compositional, structural, and functional insights.
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DOI：
10.1128/mbio.01781-14
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发表时间：
2014-09-09
期刊：
影响因子：
6.4
通讯作者：
Lopez-Ribot JL
中科院分区：
文献类型：
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作者：
Lopez-Ribot JL
在致病性真菌中，白色念珠菌最常与生物膜形成相关，这是一种完全不同于牙周炎状态的生活方式。这些生物膜的区别特征之一是存在细胞外物质，通常称为“生物膜基质”。真菌生物膜基质将固着细胞嵌入这些群落中，并发挥重要的结构和生理功能，包括具有重要临床影响的抗真菌药物耐药性。这种基质主要由真菌细胞自身产生，由不同类型的生物聚合物组成。在白色念珠菌中，生物膜基质的主要成分是碳水化合物、蛋白质、脂质和DNA，但其中许多成分仍未被鉴定和/或表征不佳。在他们最近的文章中，Zarnowski等人[mBio 5（4）：e01333-14，2014，doi：10.1128/mBio.01333-14]使用多种生物化学和最先进的“组学”方法（糖组学、蛋白质组学和脂质组学）来鉴定和表征存在于白色念珠菌生物膜基质中的独特生物聚合物。除了从每个不同的大分子类别中产生一个真正的“单足”目录的各个部分，结果还提供了重要的见解真菌生物膜基质的结构和功能方面，特别是不同组分之间的相互作用和多种基质成分对生物膜抗真菌药物耐药性的贡献。
Among pathogenic fungi, Candida albicans is most frequently associated with biofilm formation, a lifestyle that is entirely different from the planktonic state. One of the distinguishing features of these biofilms is the presence of extracellular material, commonly referred to as the “biofilm matrix.” The fungal biofilm matrix embeds sessile cells within these communities and plays important structural and physiological functions, including antifungal drug resistance with important clinical repercussions. This matrix is mostly self-produced by the fungal cells themselves and is composed of different types of biopolymers. In C. albicans, the main components of the biofilm matrix are carbohydrates, proteins, lipids, and DNA, but many of them remain unidentified and/or poorly characterized. In their recent article, Zarnowski et al. [mBio 5(4):e01333-14, 2014, doi:10.1128/mBio.01333-14] used a variety of biochemical and state-of-the-art “omic” approaches (glycomics, proteomics, and lipidomics) to identify and characterize unique biopolymers present in the C. albicans biofilm matrix. Besides generating a true “encyclopedic” catalog of individual moieties from each of the different macromolecular categories, results also provide important insights into structural and functional aspects of the fungal biofilm matrix, particularly the interaction between different components and the contribution of multiple matrix constituents to biofilm antifungal drug resistance.