The influence of surface morphology and rigidity of the substrata on cell motility
The influence of surface morphology and rigidity of the substrata on cell motility
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DOI：
10.1016/j.matlet.2009.05.073
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发表时间：
2009-08-31
影响因子：
3
通讯作者：
Yeh, Ming-Long
中科院分区：
文献类型：
--
作者：
Chen, Chien-Chang;Hsieh, Patrick Chin-Ho;Yeh, Ming-Long
本研究的目的是测量细胞在不同硬度和不同图案的基质下的运动性。本研究以人黑色素瘤细胞为研究对象。包括平面在内的三种表面填充图案。利用微电子机械系统(MEMS)在聚二甲基硅氧烷(PDMS)表面形成了6微米的m锥体和6微米的m槽。以班级培养皿为对照。细胞接种30min后，每分钟拍摄细胞图像，每组4~5个培养皿，计算出27~38个细胞。玻璃、平板、锥体的细胞运动速度分别为1.13+/-1.30、2.12+/-1.21、2.39+/-1.11和3.08+/-1.49微米/分。和Groove PDMS。PDMS组的细胞移动速度明显快于对照组(玻璃)，这是因为前者基质的硬度较小。在有沟槽的PDMS上，80%以上的细胞沿着沟槽移动，表明沟槽表面的形态可以控制细胞的运动方向。我们的结果表明，底物的模数和图案会影响细胞的运动。(C)2009爱思唯尔B.V.保留所有权利。
The purpose of this study is to measure the cell motility under different stiffness and pattern of the substrata. Human melanoma cells were used for this study. Three surface patterns including flat. 6 mu m-cone, and 6 mu m-groove on polydimethylsiloxane (PDMS) were created by the micro-electro-mechanical system (MEMS). Class dish was used as control substrate. After cells were seeded for 30 min, the cell images were taken every minute for 2 h. Each group had 4-5 dishes and 27-38 cells were calculated. The cell motility was 1.13 +/- 1.30, 2.12 +/- 1.21, 2.39 +/- 1.11 and 3.08 +/- 1.49 mu m/min on glass, flat, cone. and groove PDMS, respectively. Cells in PDMS groups moved significantly faster than the control group (glass) due to smaller stiffness of the former substrates. More than 80% of cells on grooved PDMS moved along the grooves, indicating the grooved surface morphology could control the direction of cell movement. Our results display that substrate modulus and pattern can influence cell motility. (C) 2009 Elsevier B.V. All rights reserved.