Unilocular fat cells in three-dimensional collagen gel matrix culture.
Unilocular fat cells in three-dimensional collagen gel matrix culture.
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三维胶原凝胶基质培养中的单眼脂肪细胞。
DOI：
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发表时间：
1988
影响因子：
6.5
通讯作者：
Tomoko Matsumoto
中科院分区：
文献类型：
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作者：
Hajime Sugihara;N. Yonemitsu;Shuji Tda;Shinichi Miyabara;S. Funatsumaru;Tomoko Matsumoto
最近开发的胶原凝胶三维培养技术用于在比单层培养更生理的条件下对一些哺乳动物细胞进行体外研究，该技术已应用于单眼脂肪细胞的生物学研究。成功嵌入凝胶中后，单房脂肪细胞能够保持其细胞功能并积极增殖。这些发现证实单房脂肪细胞在体外条件下确实会发生增殖，如单层培养中所证明的那样。
Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture.