Identification and expression of mangrove rivulus (Kryptolebias marmoratus) histone deacetylase (HDAC) and lysine acetyltransferase (KAT) genes
Identification and expression of mangrove rivulus (Kryptolebias marmoratus) histone deacetylase (HDAC) and lysine acetyltransferase (KAT) genes
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DOI：
10.1016/j.gene.2018.12.057
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发表时间：
2019-04-05
期刊：
影响因子：
3.5
通讯作者：
Silvestre, Frederic
中科院分区：
文献类型：
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作者：
Fellous, Alexandre;Earley, Ryan L.;Silvestre, Frederic
在配子发生和胚胎发育过程中，跨细胞/组织类型和时间的基因表达的精确调节至关重要。在脊椎动物中，转录部分受到组蛋白赖氨酸乙酰化/脱乙酰化的调节，这是一种由赖氨酸乙酰转移酶 (KAT) 和组蛋白脱乙酰酶 (HDAC) 介导的表观遗传机制。这些酶在哺乳动物中得到了很好的表征，但在斑马鱼发育之外的鱼类胚胎学中是未知的。在这里，我们表征了自体受精红树林河溪鱼 Kryptolebias marmoratus 中假定的 KAT 和 HDAC 酶，Kryptolebias marmoratus 是一种自然自体受精并可以产生同基因谱系的物种。这一独特的特征为阐明表观遗传机制作为表型可塑性来源的作用提供了机会。在这项研究中，已鉴定出 27 个 KAT 基因和 17 个 HDAC 基因。它们的保守结构域和系统发育分析表明，相对于已表征酶的其他脊椎动物，我们物种中酶的活性是保守的。此外，胚胎发生过程中、成体性腺和大脑中 KAT 和 HDAC mRNA 表达的动态，证明了其在早期和晚期发育以及雄性/雌雄配子发生和成体神经发生中的假定生物学功能。我们的研究旨在为红树林溪流自体受精鱼中可能调节组蛋白乙酰化的表观遗传因子提供基础。在脊椎动物中独一无二的是，该物种中自然存在的大量同基因谱系允许探索调节组蛋白乙酰化的酶仅对硬骨鱼繁殖和发育的贡献，这是水产养殖、生态毒理学、行为、进化、性决定论和人类疾病等基础和应用研究中非常强大的模型。
During gametogenesis and embryonic development, precise regulation of gene expression, across cell/tissue types and over time, is crucial. In vertebrates, transcription is partly regulated by histone lysine acetylation/deacetylation, an epigenetic mechanism mediated by lysine acetyltransferases (KAT) and histone deacetylases (HDAC). Well characterized in mammals, these enzymes are unknown in fish embryology outside of zebrafish development. Here, we characterized putative KAT and HDAC enzymes in the self-fertilizing mangrove rivulus fish, Kryptolebias marmoratus, a species that naturally self-fertilizes and can produce isogenic lineages. This unique feature provides an opportunity to elucidate the role of epigenetic mechanisms as a source of phenotypic plasticity. In this study, twenty-seven KAT and seventeen HDAC genes have been identified. Their conserved domains and their phylogenetic analysis suggest conservation of the enzymes' activity in our species, relative to other vertebrates in which the enzymes have been characterized. Furthermore, the dynamics of KAT and HDAC mRNA expression during embryogenesis, in adult gonads and brains, argues for a putative biological function in early and late development as well as in male/hermaphrodite gametogenesis and adult neurogenesis. Our study aimed to provide a basis about the epigenetic actors putatively regulating histone acetylation in a self-fertilizing fish, the mangrove rivulus. Unique among vertebrates, the great number of isogenic lineages occurring naturally in this species allows exploring the contribution of the enzymes regulating histone acetylation only to reproduction and development in teleost fishes, which are very powerful models in fundamental and applied researches that include aquaculture, ecotoxicology, behaviour, evolution, sexual determinism and human diseases.