Strophioblachins A-K, Structurally Intriguing Diterpenoids from Strophioblachia fimbricalyx with Potential Anticardiac Hypertrophic Inhibitory Activity
Strophioblachins A-K, Structurally Intriguing Diterpenoids from Strophioblachia fimbricalyx with Potential Anticardiac Hypertrophic Inhibitory Activity
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DOI：
10.1021/acs.jnatprod.2c01115
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发表时间：
2023-04-20
影响因子：
5.1
通讯作者：
Li，Xiao-Li
中科院分区：
文献类型：
--
作者：
Qiu，Xiong;Huang，Yong-Xiang;Li，Xiao-Li
Three new rearranged diterpenoids, strophioblachins A–C (1–3), eight new diterpenoids, strophioblachins D–K (4–11), and seven previously described diterpenoids (12–18) were purified from the aerial parts ofStrophioblachia fimbricalyx.Compounds1and2contain a rare 6/6/5/6 ring system, while3has an uncommon tricyclo[4.4.0.08,9]tridecane-bridged unit, and their diterpenoid skeletons are being reported for the first time. Utilizing spectroscopic and HRESIMS data analysis, the structures of the new compounds (1–11) were established, and ECD and13C NMR calculations were used to confirm the relative and absolute configurations of11and9. The absolute configurations of compounds1,3, and10were established using single-crystal X-ray diffraction. The results of testing for anticardiac hypertrophic activity demonstrated that compounds10and15dose-dependently lowered the mRNA expression ofNppaandNppb. Protein levels were confirmed by Western blotting, which also demonstrated that compounds10and15lowered the expression of the hypertrophic marker ANP. The cytotoxic activity against neonatal rat cardiomyocytes was assayed in vitro by the CCK-8 and ELISA methods, and the results showed that compounds10and15were only very weakly active in the range.
