Inhibition of human gelatinases by metals released from dental amalgam
Inhibition of human gelatinases by metals released from dental amalgam
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DOI：
10.1016/s0142-9612(00)00388-4
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发表时间：
2001-07-01
期刊：
影响因子：
14
通讯作者：
Line, SRP
中科院分区：
文献类型：
--
作者：
Souza, AP;Gerlach, RF;Line, SRP
金属离子与口腔环境的相互作用是牙科研究的主要课题。基质金属蛋白酶(MMPs)参与了牙周组织破坏、根面龋病、肿瘤侵袭和颞下颌关节紊乱等口腔病理过程。本研究的目的是测试从牙科银汞合金中释放出来的金属离子对主要的牙周明胶溶解MMPs的影响。将人牙龈外植体在DMEM中培养过夜，在含分散相和连续相银汞合金的缓冲液中用明胶酶谱分析分泌酶的活性。免疫沉淀鉴定条件培养液中的主要酶为基质金属蛋白酶-2和基质金属蛋白酶-9。分散相汞齐条件缓冲液对基质金属蛋白酶-2和基质金属蛋白酶-9的蛋白分解活性有较强的抑制作用。在汞合金调节缓冲液中加入二价金属螯合剂1.10邻菲咯啉，可部分阻止对基质金属蛋白酶-2和基质金属蛋白酶-9活性的抑制。牙科银汞合金条件缓冲液还能抑制纯化的基质金属蛋白酶-2对变性的I型胶原的降解。这些发现提示口腔组织MMPs的活性可能受银汞合金释放出的金属离子的调节。(C)2001爱思唯尔科学有限公司。保留所有权利。
The interaction between metal ions and the oral environment is a major subject matter in dental research. Matrix metalloproteinases (MMPs) have been implicated in pathologic oral processes such as periodontal tissue destruction, root caries, tumor invasion and temporomandibular joint disorders. The aim of this study was to test the effect of metal ions released From dental amalgam on the major gingival gelatinolytic MMPs. Gingival human explants were cultured overnight in DMEM and the activity of secreted enzymes was analyzed by gelatin zymography in buffers conditioned with dispersed phase or concentional phase dental amalgams. The major enzymes present in conditioned media were characterized as MMP-2 and MMP-9 by immunoprecipitation. The proteolytic activities of MMP-2 and MMP-9 were strongly inhibited by dispersed phase amalgams conditioned buffers. Inhibition of MMP-2 and MMP-9 activities was partly prevented by the addition of 1.10 phenanthroline, a divalent metal chelator, to the amalgam conditioned buffers. Dental amalgam conditioned buffer also inhibited the degradation of denatured type I collagen by purified MMP-2 on liquid phase assays. These findings suggest that the activity of oral tissue MMPs may be modulated by metal ions released from dental amalgam. (C) 2001 Elsevier Science Ltd. All rights reserved.