Isolation and characterization of microsatellite DNA loci from the golden cuttlefish, Sepia esculenta Hoyle (Cephalopoda)
Isolation and characterization of microsatellite DNA loci from the golden cuttlefish, Sepia esculenta Hoyle (Cephalopoda)
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金乌贼 (Sepia esculenta Hoyle)（头足类）微卫星 DNA 位点的分离和表征
DOI：
10.1111/j.1471-8286.2006.01518.x
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Taniguchi, Nobuhiko
中科院分区：
文献类型：
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作者：
Zheng, Xiaodong;Ikeda, Minoru;Taniguchi, Nobuhiko
从金乌贼Sepia esculenta Hoyle中分离出第一个微卫星标记。设计了11对引物，通过聚合酶链反应扩增标记序列。以日本爱媛县沿海沃茨的一个野生群体的45-50个个体为材料，对11个微卫星位点进行了多态性筛选。所有微卫星位点均表现多态性，每个位点的等位基因数为7 ~ 27个。观察到的和预期的杂合度范围分别为0.380至0.980和0.654至0.940。除一个位点偏离Hardy-Weinberg平衡外，其余标记位点对该物种的遗传变异和种群结构的评估都有一定的参考价值。
The first microsatellite markers were isolated from the golden cuttlefish, Sepia esculenta Hoyle. Eleven primer sets were designed to amplify the marker sequences via polymerase chain reaction. The 45-50 individuals from one wild population in the coastal waters of Ehime Prefecture, Japan were used to screen polymorphism in the 11 microsatellite loci. All the microsatellite loci were polymorphic, with the range of alleles from seven to 27 per locus. The observed and expected heterozygosities ranged from 0.380 to 0.980 and from 0.654 to 0.940, respectively. These marker loci except for one locus showing significant deviation from Hardy-Weinberg equilibrium will be useful for the assessment of genetic variation and population structure of this species.