A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos.
A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos.
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DOI：
10.1083/jcb.103.6.2649
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发表时间：
1986-12
期刊：
影响因子：
--
通讯作者：
Takeichi M
中科院分区：
文献类型：
--
作者：
Nose A;Takeichi M
钙依赖的细胞粘附分子，称为钙粘蛋白，以前分为两个亚类，E-和N-型，具有不同的粘附特异性。在这项研究中，我们确定了一类新的钙粘蛋白，称为P-钙粘蛋白，使用内脏内胚层细胞系PSA 5- E。这种钙粘蛋白是一种118，000-D的糖蛋白，在免疫特异性和分子量上不同于E-和N-钙粘蛋白。与这些发现雅阁，具有P-钙粘蛋白的细胞不与具有E-钙粘蛋白的细胞交叉粘附。P-Cadherin最早出现在小鼠胚胎发育过程中的胚外外胚层和卵柱期的内脏内胚层，后来在各种组织中表达。胎盘和子宫蜕膜表达最丰富。P-cadherin在许多组织中的表达是短暂的，并且其永久表达仅限于某些组织，如表皮、间皮和角膜内皮。当比较P-钙粘蛋白和E-钙粘蛋白的组织分布时，我们发现：每种钙粘蛋白显示出独特的时空表达模式; P-钙粘蛋白与E-钙粘蛋白在各种组织的局部区域共表达; P-钙粘蛋白表达的开始或终止与细胞层的连接或分离密切相关，与其他钙粘蛋白一样。这些结果表明，多种类型的钙粘蛋白的差异表达发挥作用，通过提供细胞的异质性粘附特异性的植入和胚胎的形态发生。
The Ca2+-dependent cell adhesion molecules, termed cadherins, were previously divided into two subclasses, E- and N-types, with different adhesive specificity. In this study, we identified a novel class of cadherin, termed P-cadherin, using a visceral endoderm cell line PSA5- E. This cadherin was a 118,000-D glycoprotein and distinct from E- and N-cadherins in immunological specificity and molecular mass. In accord with these findings, cells with P-cadherin did not cross-adhere with cells with E-cadherin. P-Cadherin first appeared in developing mouse embryos in the extraembryonic ectoderm and the visceral endoderm at the egg cylinder stage and later was expressed in various tissues. The placenta and the uterine decidua most abundantly expressed this cadherin. The expression of P-cadherin was transient in many tissues, and its permanent expression was limited to certain tissues such as the epidermis, the mesothelium, and the corneal endothelium. When the tissue distribution of P-cadherin was compared with that of E-cadherin, we found that: each cadherin displayed a unique spatio-temporal pattern of expression; P-cadherin was co-expressed with E-cadherin in local regions of various tissues; and onset or termination of expression of P- cadherin was closely associated with connection or segregation of cell layers, as found with other cadherins. These results suggested that differential expression of multiple classes of cadherins play a role in implantation and morphogenesis of embryos by providing cells with heterogenous adhesive specificity.