Pemphigus in the dog: comparison of immunofluorescence and immunoperoxidase method to demonstrate intercellular immunoglobulins in the epidermis.
Pemphigus in the dog: comparison of immunofluorescence and immunoperoxidase method to demonstrate intercellular immunoglobulins in the epidermis.
复制标题
狗的天疱疮：免疫荧光和免疫过氧化物酶方法的比较，以证明表皮中的细胞间免疫球蛋白。
DOI：
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发表时间：
1984
影响因子：
1
通讯作者：
H. Schenk
中科院分区：
文献类型：
--
作者：
M. Suter;D. Palmer;S. Zindel;H. Schenk
在3只寻常型天疱疮和4只落叶型天疱疮犬的表皮棘层中发现了细胞间免疫球蛋白。将冷乙醇固定和石蜡包埋组织切片上的免疫荧光技术与福尔马林固定石蜡包埋组织切片上的免疫过氧化物酶法进行比较。两种方法的结果完全相同。然而，未标记的抗体-酶法的优点是，相同的福尔马林固定的组织标本可用于传统的光学显微镜检查，以及免疫组织学研究。
In 3 dogs with pemphigus vulgaris and 4 dogs with pemphigus foliaceus, intercellular immunoglobulins were demonstrated in the epidermal stratum spinosum. The immunofluorescence technique on cold ethanol-fixed and paraffin-embedded tissue sections was compared with the immunoperoxidase method on formalin-fixed paraffin-embedded tissue sections. The results of both methods were identical. However, the advantage of the unlabeled antibody-enzyme method was that the same formalin-fixed tissue specimens could be used for conventional light microscopy, as well as for immunohistologic studies.