Near-infrared-emitting NaYF4: Yb, Tm/Mn upconverting nanoparticle/gold nanorod electrochemiluminescence resonance energy transfer system for sensitive prostate-specific antigen detection
Near-infrared-emitting NaYF4: Yb, Tm/Mn upconverting nanoparticle/gold nanorod electrochemiluminescence resonance energy transfer system for sensitive prostate-specific antigen detection
复制标题
近红外发射NaYF4：Yb、Tm/Mn上转换纳米粒子/金纳米棒电化学发光共振能量转移系统，用于敏感前列腺特异性抗原检测
DOI：
10.1007/s00216-017-0212-2
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发表时间：
2017-04-01
影响因子：
4.3
通讯作者：
Chen, Hongqi
中科院分区：
文献类型：
--
作者：
Gao, Ni;Ling, Bo;Chen, Hongqi
建立了检测前列腺特异性抗原(PSA)的电化学发光共振能量转移(ECL-RET)系统。以发射近红外(NIR)的NaYF4：Yb，Tm/Mn上转换纳米粒子(UCNPs)为施主，金纳米棒(GNRs)为受主。掺入Mn2+后，发光强度提高近3倍，发射峰位于808 nm处。抗PSA-1(Ab(1))经聚丙烯酸(PAA)修饰后结合到UCNPs表面。在受体方面，将十六烷基三甲基溴化铵(CTAB)封端的GNRs用11-巯基十一酸(MudA)处理，然后与抗PSA-2(Ab(2))偶联。当加入PSA时，通过抗体和抗原的特定相互作用，供体和受体被拉近了距离，导致了高猝灭效率水平。在最佳条件下，PSA的线性范围为3.75~938pg/m L(R=0.999)，检出限为3.16pg/m L。该方法用于人血清中PSA的检测，结果令人满意。
An electrochemiluminescence resonance energy transfer (ECL-RET) system that detects prostate-specific antigen (PSA) was created. Near-infrared (NIR)-emitting NaYF4: Yb, Tm/Mn upconverting nanoparticles (UCNPs) are used as donors, and gold nanorods (GNRs) are used as acceptors. The ECL was enhanced nearly threefold by Mn2+ doping, with an emission peak appearing at an NIR wavelength of 808 nm. Anti-PSA 1 (Ab(1)) was bound to the surfaces of UCNPs after being modified with poly(acrylic acid) (PAA). As for acceptors, cetyltrimethylammonium bromide (CTAB)capped GNRs were treated with 11-mercaptoundecanoic acid (MUDA) and then conjugated with Anti-PSA 2 (Ab(2)). When PSA was added, donors and acceptors were brought in close proximity through specific interactions of antibodies and antigens, resulting in high quenching efficiency levels. Under optimal conditions, the linear range of detection was 3.75-938 pg/mL for PSA (R = 0.999), with a detection limit as low as 3.16 pg/mL. This method can be applied to detect PSA in human serums with satisfactory results.