Differential colocalization of estrogen receptor β (ERβ) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain:: An immunocytochemical study
Differential colocalization of estrogen receptor β (ERβ) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain:: An immunocytochemical study
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DOI：
10.1073/pnas.95.6.3281
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Weiland, NG
中科院分区：
文献类型：
--
作者：
Alves, SE;Lopez, V;Weiland, NG
有证据表明，新发现的雌激素受体β (ER β)存在于大鼠室旁核（PVN）和视上核（SON）中，这些区域缺乏ER α。目前，我们研究是否在这些区域的几种主要神经肽系统的细胞中发现内质网β样免疫反应性（-ir）。年轻成年Sprague-Dawley大鼠卵巢切除（OVX）， 1周后一半的动物接受雌二醇-17 β (E)。使用ER β抗体对相邻切片进行双标记免疫细胞化学，然后使用催产素（OT），精氨酸-加压素（AVP）或促肾上腺皮质激素释放激素的抗体。核ER β -ir在SON和交叉后SON以及特定的PVN亚核：内侧细胞旁部分，腹侧和背侧区域，背侧和外侧细胞旁部分，以及后大细胞部分，内侧和外侧区域中被鉴定。然而，大细胞区域内的ER β -ir明显较弱。在OVX和OVX+E脑的细胞旁亚核神经元中证实了OT-/ER β -ir共定位（约50%的OT和25%的ER β -标记细胞在bregma -1.78和-2.00之间）。相比之下，PVN小细胞神经元很少含有avp和ER β -ir。作为一个整体，在含有促肾上腺皮质激素释放激素（ER β -ir）的细胞分布中观察到很少重叠。在SON中，大多数核ER β -ir与AVP-ir共定位，而很少观察到OT-/ER β -ir双标记细胞。这些发现表明，雌激素可以通过内质网β介导的机制，以组织特异性的方式直接调节特异性OT和AVP系统。
Evidence exists for the localization of the newly identified estrogen receptor beta (ER beta) within the rat paraventricular nucleus (PVN) and supraoptic nucleus (SON), regions which lack ER alpha. Presently, we investigate whether ER beta-like-immunoreactivity (-ir) is found within cells of several major neuropeptide systems of these regions. Young adult Sprague-Dawley rats were ovariectomized (OVX), and 1 week later half of the animals received estradiol-17 beta (E). Dual-label immunocytochemistry was performed on adjacent sections by using an ER beta antibody, followed by an antibody to either oxytocin (OT), arginine-vasopressin (AVP), or corticotropin releasing hormone. Nuclear ER beta-ir was identified within SON and retrochiasmatic SON, and in specific PVN subnuclei: medial parvicellular part, ventral and dorsal zones, dorsal and lateral parvicellular parts, and in the posterior magnocellular part, medial and lateral zones. However, the ER beta-ir within magnocellular areas was noticeably less intense. OT-/ER beta-ir colocalization was confirmed in neurons of the parvicellular subnuclei, in both OVX and OVX+E brains (approximate to 50% of OT and 25% of ER beta-labeled cells between bregma -1.78 and -2.00). In contrast, few PVN parvicellular neurons contained bath AVP-and ER beta-ir. As web, very little overlap aas observed in the distribution of cells containing corticotropin releasing hormone-or ER beta-ir. In the SON, most nuclear ER beta-ir colocalized with AVP-ir, whereas few OT-/ER beta-ir dual-labeled cells were observed. These findings suggest that estrogen can directly modulate specific OT and AVP systems through an ER beta-mediated mechanism, in a tissue-specific manner.