Purkinje cell activity during classical eyeblink conditioning in decerebrate guinea pigs
Purkinje cell activity during classical eyeblink conditioning in decerebrate guinea pigs
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DOI：
10.1016/j.brainres.2005.10.090
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发表时间：
2006-01-12
期刊：
影响因子：
2.9
通讯作者：
Kirino, Y
中科院分区：
文献类型：
--
作者：
Kotani, S;Kawahara, S;Kirino, Y
浦肯野细胞是小脑皮质的唯一输出，在经典的眨眼条件反射中起着关键作用。本研究首次揭示了去大脑豚鼠在连续眨眼条件反射过程中个体浦肯野细胞活动的学习相关变化，这允许从小脑皮质的单叶连续进行单一单位记录。条件刺激组接受条件刺激(CS)和非条件刺激(US)的配对呈现，直到条件反应(CR)的频率超过80%。对照组以伪随机方式收到数量相当的CS和US。浦肯野细胞对CS的反应分为四种类型：兴奋性、抑制性、两者结合或无反应。两组记录的细胞中大约有一半在不同的条件化阶段改变了它们的反应类型。浦肯野细胞对CS的放电频率在对条件作用组有降低的趋势，而在假条件作用组有升高的趋势。这种与学习相关的反应类型变化的差异可归因于NO反应和抑制性反应类型的变化的差异。对神经活动和认知障碍时间模式的相关分析表明，通过配对条件反射产生抑制反应的细胞大多获得了认知障碍相关的时间模式。这些结果表明，与学习相关的浦肯野细胞获得了一种抑制反应，其时间模式与CR地形图相关。(C)2005 Elsevier B.V.保留所有权利。
Purkinje cells are the sole output from the cerebellar cortex and play a critical role during classical eyeblink conditioning. The present study revealed for the first time a learning-related change in individual Purkinje cell activity during successive eyeblink conditioning in decerebrate guinea pigs which permitted continuous single unit recording from the simplex lobe of the cerebellar cortex. The pair-conditioned group received paired presentation of the conditioned stimulus (CS) and unconditioned stimulus (US) until the frequency of the conditioned response (CR) exceeded 80%. The control group received a comparable number of the CS and US in a pseudorandom fashion. Responses of Purkinje cells to the CS were classified into four types: excitatory, inhibitory, a combination of the two, or no response. Approximately half of the recorded cells from both groups changed their response type at various conditioning stages. The firing frequency of a Purkinje cell to the CS showed a tendency to decrease in the pair-conditioned group, while it had a tendency to increase in the pseudoconditioned group. This learning-related difference in change of response type was attributable to a difference in the change between the no response and the inhibitory response types. Correlation analysis of the temporal pattern between the neural activity and the CR revealed that most of the cells that developed an inhibitory response by paired conditioning acquired the CR-related temporal pattern. These results suggest that the learning-related Purkinje cells gain an inhibitory response with a temporal pattern correlated with the CR topography. (c) 2005 Elsevier B.V. All rights reserved.