Natural variation in ZmFBL41 confers banded leaf and sheath blight resistance in maize
Natural variation in ZmFBL41 confers banded leaf and sheath blight resistance in maize
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ZmFBL41 的自然变异赋予玉米带状叶枯病和纹枯病抗性
DOI：
10.1038/s41588-019-0503-y
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发表时间：
2019-10-01
期刊：
影响因子：
30.8
通讯作者：
Chu, Zhaohui
中科院分区：
文献类型：
--
作者：
Li, Ning;Lin, Bao;Chu, Zhaohui
立枯丝核菌（Rhizoctonia solani）是一种广泛分布的植物病原菌，可引起玉米的条纹叶枯病和水稻的纹枯病。在这里，我们确定了一个F盒蛋白（ZmFBL 41），赋予抗条纹叶枯病和纹枯病通过全基因组关联研究在玉米。过量表达ZmFBL 41的水稻对稻瘟病菌的敏感性提高。索拉尼该等位基因中的两个氨基酸取代阻止其与ZmCAD的相互作用，ZmCAD编码单纤维素生物合成途径中的最终酶，导致ZmCAD降解的抑制，并因此导致木质素的积累和损伤扩展的限制。敲除水稻ZmCAD同源基因OsCAD8B可增强水稻对稻曲病的感病性。索拉尼结果揭示了一种敏感机制，其中R。solani靶向宿主蛋白酶体以改变植物细胞壁的次级代谢以用于其入侵。更重要的是，它提供了一个产生R。不同植物品种的抗茄病品种。
Rhizoctonia solani is a widely distributed phytopathogen that causes banded leaf and sheath blight in maize and sheath blight in rice. Here, we identified an F-box protein (ZmFBL41) that confers resistance to banded leaf and sheath blight through a genome-wide association study in maize. Rice overexpressing ZmFBL41 showed elevated susceptibility to R. solani. Two amino acid substitutions in this allele prevent its interaction with ZmCAD, which encodes the final enzyme in the monolignol biosynthetic pathway, resulting in the inhibition of ZmCAD degradation and, consequently, the accumulation of lignin and restriction of lesion expansion. Knocking out the ZmCAD-homologous gene OsCAD8B in rice enhanced susceptibility to R. solani. The results reveal a susceptibility mechanism in which R. solani targets the host proteasome to modify the secondary metabolism of the plant cell wall for its invasion. More importantly, it provides an opportunity to generate R. solani-resistant varieties of different plant species.