Rapid methods for determination of fluoxetine in pharmaceutical formulations
Rapid methods for determination of fluoxetine in pharmaceutical formulations
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DOI：
10.1016/s0731-7085(02)00154-1
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发表时间：
2002-08-01
影响因子：
3.4
通讯作者：
Raggi, MA
中科院分区：
文献类型：
--
作者：
Mandrioli, R;Pucci, V;Raggi, MA
两种不同的分析方法的氟西汀在商业配方的质量控制已经开发和比较：荧光分光光度法和毛细管区带电泳（CZE）方法。当在λ = 230 nm激发时，在λ = 293 nm测量荧光发射值。CZE方法使用未涂覆的熔融石英毛细管和pH 2.5磷酸盐缓冲液作为背景电解质。从胶囊中提取氟西汀包括用甲醇/水溶解、过滤和稀释的简单一步。两种方法在精密度方面均获得了令人满意的结果，电泳法获得了最佳结果，RSD%值始终低于2.0%。通过回收率研究评估了准确度，结果非常好，在97.5%和102.6%之间。此外，这两种方法都具有非常快速的优点。(C)2002 Elsevier Science B. V.保留所有权利。
Two different analytical methods for the quality control of fluoxetine in commercial formulations have been developed and compared: a spectrofluorimetric method and a capillary zone electrophoretic (CZE) method. The fluorescence emission values were measured at lambda = 293 nm when exciting at lambda = 230 nm. The CZE method used an uncoated fused-silica capillary and pH 2.5 phosphate buffer as the background electrolyte. The extraction of fluoxetine from the capsules consisted of a simple one-step dissolution with methanol/water, filtration and dilution. Both methods gave satisfactory results in terms of precision the best results were obtained for the electrophoretic method, with RSD% values always lower than 2.0%. The accuracy was assessed by means of recovery studies, which gave very good results, between 97.5 and 102.6%. Furthermore, both methods also have the advantage of being very rapid. (C) 2002 Elsevier Science B.V. All rights reserved.