Diminished sympathetic nervous system activity in genetically obese (ob/ob) mouse.
Diminished sympathetic nervous system activity in genetically obese (ob/ob) mouse.
复制标题
遗传性肥胖 (ob/ob) 小鼠的交感神经系统活动减弱。
DOI：
10.1152/ajpendo.1983.245.2.e148
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Landsberg,L
中科院分区：
文献类型：
--
作者：
Young,JB;Landsberg,L
遗传性肥胖（ob/ob）小鼠对冷暴露表现出有缺陷的体温调节反应。这种现象的病理生理学解释集中在细胞内代谢异常或外周组织对儿茶酚胺的产热作用不敏感。由于交感神经系统（SNS）是受反馈调节，外周损害产热应与SNS活动的代偿性增加。为了检查ob/ob小鼠中的SNS活性，在ob/ob和瘦小鼠的心脏和肩胛间棕色脂肪组织（IBAT）中测量去甲肾上腺素（NE）周转。利用放射性标记的NE或用α-甲基-p-酪氨酸抑制NE生物合成来测量NE周转的研究结果表明，与瘦对照组的测量结果相比，在环境温度（22 ℃）下，ob/ob小鼠心脏中SNS活性降低33-56%，IBAT活性降低45-73%。在冷暴露（4摄氏度）NE营业额增加心脏和IBAT在ob/ob和瘦小鼠相似的程度，但NE营业额率在心脏，可能在IBAT以及，保持较低的肥胖小鼠比在瘦尽管在ob/ob小鼠在此期间逐渐发展的体温过低。纳洛酮（一种长效阿片拮抗剂）的给药未能逆转在ob/ob小鼠中观察到的SNS活性抑制。这些数据表明，减少SNS活动在ob/ob小鼠可能是一个额外的因素，这些动物的产热缺陷的特点。
The genetically obese (ob/ob) mouse exhibits defective thermoregulatory responses to cold exposure. Pathophysiological explanations for this phenomenon have focused on abnormalities in intracellular metabolism or insensitivity of peripheral tissues to the thermogenic effects of catecholamines. Because the sympathetic nervous system (SNS) is subject to feedback regulation, a peripheral impairment in thermogenesis should be associated with a compensatory increase in SNS activity. To examine SNS activity in the ob/ob mouse, norepinephrine (NE) turnover was measured in heart and interscapular brown adipose tissue (IBAT) of ob/ob and lean mice. The results from studies utilizing radiolabeled NE or inhibition of NE biosynthesis with alpha-methyl-p-tyrosine to measure NE turnover demonstrated reductions in SNS activity of 33-56% in heart and of 45-73% in IBAT in ob/ob mice at ambient temperature (22 degrees C) compared with measurements in lean controls. During cold exposure (4 degrees C) NE turnover increased in heart and IBAT to a similar extent in both ob/ob and lean mice, but NE turnover rates in heart, and probably in IBAT as well, remained lower in the obese mice than in the lean despite the gradual development of hypothermia in the ob/ob mice during this period. Administration of naltrexone, a long-acting opiate antagonist, failed to reverse the suppression of SNS activity observed in the ob/ob mice. These data indicate that diminished SNS activity in ob/ob mice may be an additional factor contributing to the defective thermogenesis characteristic of these animals.