Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristic in peri-operative esophageal cancer patients
Multipoint quantification of multimarker genes in peripheral blood and micrometastasis characteristic in peri-operative esophageal cancer patients
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食管癌围术期患者外周血多标志物基因及微转移特征的多点定量
DOI：
10.1016/j.canlet.2007.11.001
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发表时间：
2008-03-08
期刊：
影响因子：
9.7
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Liu, Zhian;Jiang, Ming;Ju, Huangxian
本工作提出了一种基于实时半定量RT-PCR多标记基因多点定量的方法来评估围手术期外周血（PB）中隐匿性微转移的特征。检测36例食管鳞癌（EC）患者术前（B - 1）、术后即刻（B 0）和术后第3天（B + 3）外周血SCC、CK 19、CK 20、CEA和Survivin mRNA的表达水平。SCC和CK 19 mRNA的阳性率较低，而CEA和Survivin mRNA在B - 1、B 0和B + 3的阳性率分别为58.3%、83.3%和72.3%。与CEA细胞在B - 1 ~ B 0和B 0 ~ B + 3期显著增加和缓慢减少相反，Survivin细胞在这两个时期显著减少和迅速增加。随访1.2年发现，CEA细胞B + 3/B 0比值>= 0.3或/和Survivin细胞>= 10的患者发生转移的可能性明显增高，CEA和Survivin mRNA联合检测预测发生转移的敏感性由单独检测CEA mRNA的54.5%提高到72.7%。提示CEA和Survivin基因联合检测可提高预测复发和鉴别微转移的敏感性。(C)2008年由Elsevier爱尔兰有限公司出版。
This work proposed a method to assess the occult micrometastasis characteristic in peri-operative peripheral blood (PB) based on multipoint quantification of multimarker genes by real-time semiquantitative RT-PCR. The expression levels of SCC, CK19, CK20, CEA and survivin mRNA in PB samples collected before surgery (B - 1), immediately after surgery (B0) and at the third day post-operatively (B + 3) from 36 squamous esophageal cancer (EC) patients were detected. SCC and CK19 mRNA showed low positivity detection rates, while the rate for CEA and survivin mRNA panel was 58.3%, 83.3% and 72.3% at B - 1, B0 and B + 3, respectively. Opposite to the significant increase and slow decrease of CEA cells at stages of B - 1 to B0 and B0 to B + 3, respectively, survivin cells decreased significantly and increased quickly at the two stages. The follow-up with a period of 1.2 years showed that the patients with the B + 3/B0 ratios of >= 0.3 for CEA cells or/and >= 10 for survivin cells exhibited significantly high possibility of developed metastasis, and the sensitivity to predict developed metastasis increased from 54.5% of CEA mRNA alone to 72.7% of CEA and survivin mRNA panel. These results suggested that CEA and survivin gene panel improved the sensitivity to predict recurrence and differentiate the micrometastatic process. (C) 2008 Published by Elsevier Ireland Ltd.