EBV-positive human sera contain antibodies against the EBV BMRF-2 protein.
EBV-positive human sera contain antibodies against the EBV BMRF-2 protein.
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EBV 阳性人血清含有针对 EBV BMRF-2 蛋白的抗体。
DOI：
10.1016/j.virol.2009.07.025
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Tugizov,SharofM
中科院分区：
文献类型：
--
作者：
Xiao,Jianqiao;Palefsky,JoelM;Herrera,Rossana;Sunshine,Carl;Tugizov,SharofM
We previously showed that the EBV glycoprotein BMRF-2 contains a functional integrin-binding Arg-Gly-Asp (RGD) domain that plays an important role in viral infection and cell-to-cell spread of progeny virions in oral epithelial cells. In this study, we found that EBV-seropositive human sera contain antibodies against BMRF-2. The inhibitory effect of EBV-positive sera on EBV infection of oral epithelial cells was substantially reduced by pre-incubation of serum samples with the BMRF-2 RGD peptide, suggesting that anti-BMRF-2 human antibodies possess neutralizing activity. EBV-specific sera reacted strongly with the BMRF-2 extracellular domain (170–213 aa) containing the RGD motif, whereas they reacted only weakly or not at all with a mutated form of the BMRF-2 extracellular domain containing AAA instead of RGD. These data indicate that RGD motif of BMRF-2 is part of an immunodominant antigenic determinant within the extracellular domain of BMRF-2 that may contribute to EBV neutralization during EBV reactivation.
