EXISTENCE OF A NEW-PROTEIN COMPONENT WITH THE SAME FUNCTION AS THE LUKF COMPONENT OF LEUKOCIDIN OR GAMMA-HEMOLYSIN AND ITS GENE IN STAPHYLOCOCCUS-AUREUS P83
EXISTENCE OF A NEW-PROTEIN COMPONENT WITH THE SAME FUNCTION AS THE LUKF COMPONENT OF LEUKOCIDIN OR GAMMA-HEMOLYSIN AND ITS GENE IN STAPHYLOCOCCUS-AUREUS P83
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DOI：
10.1016/0014-5793(94)01372-8
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发表时间：
1995-01-09
期刊：
影响因子：
3.5
通讯作者：
KAMIO, Y
中科院分区：
文献类型：
--
作者：
CHOORIT, W;KANEKO, J;KAMIO, Y
金黄色葡萄球菌（Staphylococcus aureus）毒素（leukocidin）和γ-溶血素（gamma-hemolysin）由两种蛋白质组分组成，即杀白细胞素的LukF和LukS以及γ-溶血素的H γ I和H γ II。该组分显示出与LukF组分对杀白细胞素或γ-溶血素的H γ I组分与LukS或H γ II组合的生物活性相同的生物活性。然而，LukM组分与抗LukS抗体交叉反应，但不与抗LukF抗体交叉反应。在对金黄色葡萄球菌P83的LukM组分进行化学分析和LukM基因的克隆及核苷酸序列测定的基础上，我们证明了LukM是一种全新的杀白细胞素或γ-溶血素的蛋白组分。LukM基因推导的氨基酸序列与LukS和HyII的同源性分别为66.7%和67%。然而，LukM和LukF的氨基酸序列显示只有29%的同源性。
Staphylococcal toxins, leukocidin and gamma-hemolysin, consist of two protein components, i.e. LukF and LukS for leukocodin and H gamma I and H gamma II for gamma-hemolysin, From a culture fluid of Staphylococcus aureus strain P83, a new protein component of leukocidin or gamma-hemolysin which was designated as LukM was isolated. This component showed the same biological activity as that of LukF component for leukocidin or H gamma I component of gamma-hemolysin in combination with LukS or H gamma II. However, the LukM component cross-reacted with the anti-LukS antibodies but not with the anti-LukF antibodies. On the basis of chemical analysis of the LukM component and the cloning and nucleotide sequencing of the lukM gene of S, aureus P83, we have demonstrated that LukM is an entirely new protein component of leukocidin or gamma-hemolysin. The deduced amino acid sequence of LukM from the lukM gene shelved 66.7% and 67% indentity to that of LukS and HyII, respectively. However, the amino acid sequence of LukM and LukF showed only 29% homology.