A Novel Approach for Detecting an Immunodominant Antigen of Porphyromonas gingivalis in Diagnosis of Adult Periodontitis
A Novel Approach for Detecting an Immunodominant Antigen of Porphyromonas gingivalis in Diagnosis of Adult Periodontitis
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检测牙龈卟啉单胞菌免疫显性抗原诊断成人牙周炎的新方法
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Okada
中科院分区：
文献类型：
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作者：
T. Kawai;Hiro;N. Sakato;H. Okada
摘要在成人牙周炎中牙龈卟啉单胞菌的长期感染过程中，会产生针对该微生物的特异性抗体反应。我们描述了一种潜在的新方法，用于识别人类牙周炎患者的免疫显性抗原。首先，从用牙龈卟啉单胞菌FDC 381的粗抗原制剂免疫的小鼠建立各种单克隆抗体（MAb）。这些单克隆抗体的抗原特异性进行了比较，与10个牙周炎患者的血清抗体在竞争性酶联免疫吸附试验。一种单克隆抗体（称为PF 18）的结合很容易被所有患者的血清抑制，但不被健康志愿者的血清抑制。免疫电子显微镜分析显示，PF 18识别的抗原存在于细胞表面，推测在被膜层中。用单克隆抗体通过免疫亲和层析进行抗原物质的纯化，称为PF 18-Ag。PF 18-Ag抗原表位的结构分析表明，PF 18-Ag抗原表位是由糖类物质组成的，而不是由肽类物质组成的。测定血清PF 18-Ag抗体水平比测定牙龈卟啉单胞菌抗原粗品抗体更能区分牙周炎患者和健康人。抗PF 18-Ag抗体以免疫球蛋白G2型为主。提示PF 18-Ag可能是一种新的抗原物质，是一种重要的抗原物质，对成人牙周炎的临床诊断具有潜在的应用价值。所使用的方法也将与检测其他感染性微生物的免疫显性抗原相关。
ABSTRACT In the course of long-term infection with Porphyromonas gingivalis in adult periodontitis, a specific antibody response to this organism is generated. We describe a potential novel approach for identifying an immunodominant antigen in human periodontitis patients. First, various monoclonal antibodies (MAbs) were established from mice immunized with crude antigen preparations of P. gingivalis FDC 381. The antigen specificities of these MAbs were compared with those of serum antibodies of 10 periodontitis patients in a competitive enzyme-linked immunosorbent assay. The binding of one MAb (termed PF18) was readily inhibited by sera from all patients but not by sera from healthy volunteers. The antigen recognized by PF18 existed on the cell surface, presumably in the capsule layer, shown by immunoelectron microscopic analysis. Purification of the antigenic substance, termed PF18-Ag, was performed by immunoaffinity chromatography with the MAb. Characterization of PF18-Ag suggested that the epitope was composed of carbohydrates but not peptides and that the substance was different from lipopolysaccharide. Measurement of levels of serum antibody to PF18-Ag better discriminated periodontitis patients from healthy individuals than measurement of antibodies to crude antigen preparations of P. gingivalis. Immunoglobulin G2 was the predominant isotype among the antibodies to PF18-Ag in the patients’ sera. These results suggest that PF18-Ag, which is possibly a novel substance, is an important antigenic substance and is potentially useful for the clinical diagnosis of adult periodontitis. The approach that was used would also be relevant to detecting immunodominant antigens of other infectious microorganisms.
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从牙龈卟啉单胞菌中裂解激肽原和纤维蛋白原的有效 70 kDa 硫醇赖氨酰蛋白酶 (Lys-gingivain) 的纯化和表征。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Scott,CF;Whitaker,EJ;Hammond,BF;Colman,RW
通讯作者：
Colman,RW
影响因子：
15.9
作者：
Cutler, Christopher W.;Kalmar, John R.;Genco, Caroline A.
通讯作者：
Genco, Caroline A.
抑制 C3 和 IgG 蛋白水解可增强牙龈卟啉单胞菌的吞噬作用。
DOI：
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发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Cutler,CW;Arnold,RR;Schenkein,HA
通讯作者：
Schenkein,HA
牙周炎患者的体液反应：治疗效果和疫苗前景。
DOI：
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发表时间：
1994
期刊：
Compendium (Newtown, Pa.). Supplement
影响因子：
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作者：
Page,RC
通讯作者：
Page,RC
影响因子：
2.9
作者：
BLAKE, MS;JOHNSTON, KH;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC