Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.
Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.
复制标题
P700 的 g 张量测量。
DOI：
10.1073/pnas.90.20.9485
复制
发表时间：
1993
影响因子：
11.1
通讯作者：
Griffin,RG
中科院分区：
文献类型：
--
作者：
Prisner,TF;McDermott,AE;Un,S;Norris,JR;Thurnauer,MC;Griffin,RG
报道了P700+的高场连续波EPR谱。在从重氮化蓝藻(聚球藻)获得的制剂中。用光系统I(PS-I)制剂、在2H2O中生长的蓝藻全细胞、光系统II(PS-II)制剂以及质子化PS-I制剂进行测量。由于我们的140 GHz能谱仪的分辨率有了显著提高(与X或Q波段EPR相比)，所以我们可以得到初级施主P700+的g张量的主值。可高精度分辨和测量，G11=2.00304，G22=2.00262，G33=2.00232。来自Mn2+的其他信号和来自PS-II在g约2.00266处的暗信号与P700+有所区别。G张量粉末状。将测得的g值与几种细菌反应中心供体的g值进行了比较。
We report high-field continuous wave EPR spectra of P700+. in preparations obtained from deuterated cyanobacteria (Synechococcus lividus). Measurements were performed with photosystem I (PS-I) preparations, whole cells from cyanobacteria grown in 2H2O, and photosystem II (PS-II) preparations, as well as with protonated PS-I preparations. Because of the significantly improved resolution of our 140-GHz spectrometer (as compared with X- or Q-band EPR) the principal values of the g-tensor of the primary donor P700+. could be resolved and measured with high accuracy as g11 = 2.00304, g22 = 2.00262, and g33 = 2.00232. Other signals arising from Mn2+ and a dark signal from PS-II at g approximately 2.00266 are distinguished from the P700+. g-tensor powder pattern. The measured g values are compared with those of several bacterial reaction center donors.