How does column packing microstructure affect column efficiency in liquid chromatography?
How does column packing microstructure affect column efficiency in liquid chromatography?
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DOI：
10.1016/j.chroma.2006.05.066
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发表时间：
2006-09
期刊：
影响因子：
--
通讯作者：
M. Schure;R. Maier
中科院分区：
文献类型：
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作者：
M. Schure;R. Maier
本文报道了全三维计算机模拟无孔色谱填料的流体流动和分散特性。填隙孔隙率和填料缺陷是不同的，试图了解的填料微观结构的色谱后果。示踪剂区的分散计算的形式板的高度作为流体速度的函数为七个模型的颗粒包，其中颗粒被选择性地从包在不同的大小和拓扑结构的集群。为了检验松散但随机填料的后果，在0.36≤ λ ≤0.50的范围内模拟了7个无缺陷填料的速度和区域分散，其中λ是间隙孔隙度。结果表明，无缺陷的松散填料可以提供良好的色谱效率，但效率可能会有所不同，这取决于微妙的细节包。当缺陷数量增加时，区域色散也相应增加。对于从λ =0.36填料中除去6%颗粒的颗粒填料，损失了约33%的柱效率。这些结果表明，在柱填充中，防止导致不均匀填充的缺陷位置比获得具有最小间隙空隙体积的最高密度填充重要得多。
Full three-dimensional computer simulations of the fluid flow and dispersion characteristics of model nonporous chromatographic packings are reported. Interstitial porosity and packing defects are varied in an attempt to understand the chromatographic consequences of the packing microstructure. The tracer zone dispersion is calculated in the form of plate height as a function of fluid velocity for seven model particle packs where particles are selectively removed from the packs in clusters of varying size and topology. In an attempt to examine the consequences of loose but random packs, the velocities and zone dispersion of seven defect-free packs are simulated over the range 0.36≤ɛ≤0.50, where ɛ is the interstitial porosity. The results indicate that defect-free loose packings can give good chromatographic efficiency but the efficiency can vary depending on subtle details of the pack. When the defect population increases, the zone dispersion increases accordingly. For a particle pack where 6% of the particles are removed from an ɛ=0.36 pack, ≈ 33% of the column efficiency is lost. These results show that it is far more important in column packing to prevent defect sites leading to inhomogeneous packing rather than obtaining the highest density pack with the smallest interstitial void volume.