Relationships of Ceratocystis fimbriata isolates from Colombian coffee-growing regions based on molecular data and pathogenicity
Relationships of Ceratocystis fimbriata isolates from Colombian coffee-growing regions based on molecular data and pathogenicity
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DOI：
10.1046/j.1439-0434.2003.00738.x
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发表时间：
2003-08-01
影响因子：
1.5
通讯作者：
Wingfield, MJ
中科院分区：
文献类型：
--
作者：
Marin, M;Castro, B;Wingfield, MJ
降低哥伦比亚咖啡种植园盈利能力的最重要疾病之一是咖啡树溃疡病。这种由毛角囊藻引起的疾病近年来严重程度和发病率有所增加，给资源匮乏的农民造成了巨大损失。本次调查的目的是考虑来自哥伦比亚不同咖啡种植区的 C. fimbriata 分离株的相关性，从而为未来开发抗性咖啡品种奠定基础。根据形态和致病性对来自 11 个省份的 50 个分离株进行了鉴定。此外，还使用限制性片段长度多态性 (RFLP) 和随机扩增多态性 DNA (RAPD) 标记来比较分离株。还对内部转录间隔区 (ITS)、Mat-2 HMG Box 和部分 β-微管蛋白基因的 DNA 序列进行了比较。所有比较的结果清楚地表明，哥伦比亚的 C. fimbriata 分离株差异很大。用于这些比较的所有分子参数还表明，来自哥伦比亚的 C. fimbriata 分离株属于两个主要的系统发育谱系。我们认为，其中一个谱系代表 C. fimbriata sensu stricto，另一个谱系代表一个需要进一步表征的新分类群。分离株对咖啡具有不同程度的致病性，其中一些导致超过 90% 的死亡，另一些则杀死 < 5% 的接种植物。咖啡种植区内致病性最强的菌株也有明显的分布模式。
One of the most important diseases that reduce the profitability of coffee plantations in Colombia is coffee tree canker. This disease, caused by Ceratocystis fimbriata, has increased in severity and incidence in recent years, resulting in substantial losses to resource-poor farmers. The aim of this investigation was to consider the relatedness of C. fimbriata isolates from different Colombian coffee-growing regions, and thus to provide a foundation for future development of resistant coffee varieties. Fifty isolates from 11 provinces were characterized based on morphology and pathogenicity. In addition, isolates were compared using restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNA (RAPD) markers. DNA sequence comparisons for the internal transcribed spacer (ITS) region, Mat-2 HMG Box and partial beta-tubulin gene, were also made. The results of all comparisons clearly showed that Colombian isolates of C. fimbriata are highly variable. All molecular parameters used for these comparisons also showed that C. fimbriata isolates from Colombia reside in two major phylogenetic lineages. We believe that one of these lineages represent C. fimbriata sensu stricto and the other, a new taxon that needs further characterization. Isolates had different levels of pathogenicity on coffee, with some causing death of more that 90% and others killing < 5% of inoculated plants. There was also a clear pattern of distribution of the most pathogenic strains within coffee-growing areas.