A casein kinase I isoform is required for proper cell cycle progression in the fertilized mouse oocyte.
A casein kinase I isoform is required for proper cell cycle progression in the fertilized mouse oocyte.
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发表时间：
1997-12
影响因子：
4
通讯作者：
S. D. Gross;C. Simerly;G. Schatten;R. Anderson
中科院分区：
文献类型：
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作者：
S. D. Gross;C. Simerly;G. Schatten;R. Anderson
酪蛋白激酶I是所有真核生物共有的丝氨酸/苏氨酸蛋白激酶家族。在酵母中，酪蛋白激酶I同源物与生长、DNA修复和细胞分裂的调节有关。此外，它们在膜结构和细胞核中的亚细胞定位对于功能是必不可少的。在高等真核生物中，存在七种遗传上不同的同种型：（alpha）、ss、（gamma）1、（gamma）2、（gamma）3、（delta）和（gamma）。酪蛋白激酶I（α）表现出细胞周期依赖性的亚细胞定位，包括在间期与胞质泡状结构和细胞核以及在有丝分裂期间与纺锤体的结合。酪蛋白激酶I还显示调节哺乳动物细胞中生长和DNA合成/修复的关键调节因子，如SV 40大T抗原和p53。这些结果表明，酪蛋白激酶I可能参与的过程类似于那些归因于酵母酪蛋白激酶I同源物。为了确定酪蛋白激酶I（α）在细胞周期调控中的作用，利用小鼠卵母细胞，因为其细胞周期明确且易于显微操作。从减数分裂I成熟到第一次合子分裂的免疫荧光研究表明，该激酶与以前报道的结构相似。在中期II停滞和G2期显微注射酪蛋白激酶I（α）抗体对第二次减数分裂或第一次分裂的完成没有影响。然而，在S期发作之前的早期原核阶段期间显微注射这些抗体阻断了激酶摄取到原核中，并干扰了正确和及时的细胞周期进展到第一次分裂。这些结果表明，在第一个细胞周期期间，激酶调节从间期到有丝分裂的进程。
Casein kinase I is a family of serine/threonine protein kinases common to all eukaryotes. In yeast, casein kinase I homologues have been linked to the regulation of growth, DNA repair and cell division. In addition, their subcellular localization to membraneous structures and the nucleus is essential for function. In higher eukaryotes, there exist seven genetically distinct isoforms: (alpha), ss, (gamma)1, (gamma)2, (gamma)3, (delta) and (epsilon). Casein kinase I(alpha) exhibits a cell cycle-dependent subcellular localization including an association with cytosolic vesicular structures and the nucleus during interphase, and the spindle during mitosis. casein kinase I has also been shown to modulate critical regulators of growth and DNA synthesis/repair in mammalian cells such as SV40 large T antigen and p53. These results suggest that casein kinase I may be involved in processes similar to those ascribed to the yeast casein kinase I homologues. To define a role for casein kinase I(alpha) in cell cycle regulation, the mouse oocyte was utilized because of its well-defined cell cycle and ease of micromanipulation. Immunofluorescence studies from meiosis I of maturation to the first zygotic cleavage demonstrated that the kinase was associated with structures similar to those previously reported. Microinjection of casein kinase I(alpha) antibodies at metaphase II-arrest and G2 phase, had no effect on the completion of second meiosis or first division. However, microinjection of these antibodies during the early pronucleate phase prior to S-phase onset blocked uptake of the kinase into pronuclei and interfered with proper and timely cell cycle progression to first cleavage. These results suggest that the kinase regulates the progression from interphase to mitosis during the first cell cycle.