Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.
Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.
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DOI：
10.1534/g3.115.020974
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发表时间：
2015-10-13
期刊：
影响因子：
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通讯作者：
Borkovich KA
中科院分区：
文献类型：
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作者：
Cabrera IE;Pacentine IV;Lim A;Guerrero N;Krystofova S;Li L;Michkov AV;Servin JA;Ahrendt SR;Carrillo AJ;Davidson LM;Barsoum AH;Cao J;Castillo R;Chen WC;Dinkchian A;Kim S;Kitada SM;Lai TH;Mach A;Malekyan C;Moua TR;Torres CR;Yamamoto A;Borkovich KA
G蛋白偶联受体（GPCR）调节真核生物（包括丝状真菌）的生长、发育和环境感知。在这些生物体中最大的预测GPCR类是Pth 11相关的，其成员类似于植物病原体Magnaporthe pasta中疾病所需的蛋白质。然而，Pth 11相关类尚未在任何丝状真菌物种中进行功能研究。在这里，我们分析了可用的突变体的表型为36 GPCR基因，包括20 Pth 11相关的，在模型丝状真菌粗糙脉孢菌。我们还研究了现有数据集中所有43个预测GPCR基因的基因表达模式。共有17个突变体（47%）具有至少一种生长或发育表型。我们确定了18个突变体（56%）具有化学敏感性或营养表型（11个独特），使至少有一个缺陷的突变体总数达到28个（78%），包括15个突变体（75%）在Pth 11相关类。GPCR基因的基因表达趋势与许多突变体观察到的表型相关，也表明几组共转录基因的功能重叠。Pth 11相关类的几个成员具有表型和/或在纤维素上差异表达，这表明该基因家族在植物细胞壁传感或利用中可能起作用。
G protein−coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization.