Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface
Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface
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DOI：
10.1099/jmm.0.46810-0
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Honda, Takeshi
中科院分区：
文献类型：
--
作者：
Ueta, Mayumi;Iida, Tetsuya;Honda, Takeshi
表皮葡萄球菌是眼表正常细菌植物群的一部分。从健康志愿者的结膜囊、上下眼睑缘和上下睑板腺获得的细菌分离株的染色体DNA进行Smal消化和PFGE以研究生物体的遗传多样性。还检查了多个菌落的S。表皮来源于相同受试者的结膜囊。最后，每月一次从四名健康受试者的眼表中收获共生细菌，持续6个月，并对S.分析了表皮isclates。结果发现，不仅来自不同受试者的细菌菌株，而且来自同一受试者的多个眼表部位的细菌菌株也表现出不同的PFGE模式。在42名受试者中，有5名受试者存在多个S.从结膜囊中分离表皮细胞; 3个具有不同PFGE模式的多个菌落，2个表现为具有相同PFGE模式的多个菌落。S. 6个月内从同一受试者的结膜囊分离的表皮表现出不同的PFGE模式。这些数据表明了S.健康眼表的表皮。
Staphylococcus epidermidis is part of the normal bacterial flora on the ocular surface. The chromosomal DNA of bacterial isolates obtained from the conjunctival sac, upper and lower lid margins, and upper and lower Meibomian glands of healthy volunteers was subjected to Smal digestion and PFGE to study the genetic diversity of the organisms. Multiple colonies were also examined of S. epidermidis derived from the conjunctival sac of the same subjects. Lastly, commensal bacteria were harvested from the ocular surfaces of four healthy subjects once a month for 6 months, and the genetic background of the S. epidermidis isclates was analysed. It was found that bacterial strains not only from different subjects but also from multiple ocular surface sites of the same subject exhibited different PFGE patterns. In five of 42 subjects multiple colonies of S. epidermidis were isolated from the conjunctival sac; three harboured multiple colonies with different PFGE patterns, and two manifested multiple colonies with identical PFGE patterns. S. epidermidis isolated from the conjunctival sac of the same subjects over a 6-month period exhibited varying PFGE patterns. The data demonstrate the polyclonality of S. epidermidis on the healthy ocular surface.