Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway
Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway
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DOI：
10.1002/cbdv.202301383
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发表时间：
2024-01-25
影响因子：
2.9
通讯作者：
Li，Bin
中科院分区：
文献类型：
--
作者：
Huang，Xiaowen;Lei，Shanshan;Li，Bin
淫羊藿属植物广泛用于促进骨愈合，其活性成分为淫羊藿总黄酮(TFE)。RAS同源基因家族成员A/Rho相关蛋白激酶(RhoA/Rock)是调节细胞骨架的重要途径，已被证明影响骨形成。然而，TFE是否通过这一途径促进骨愈合仍不清楚。本研究通过显微计算机断层扫描和病理切片苏木精-伊红染色来评价TFE的疗效。成骨细胞F-肌动蛋白染色观察TFe对细胞骨架的保护作用。通过RT-qPCR和Western印迹分析，探讨其对RhoA/ROCK1通路的调控作用。采用流式细胞术、碱性磷酸酶染色和结节钙化染色评价其对成骨的影响。TFE可促进大鼠骨愈合，减轻成骨细胞的细胞骨架损伤，下调RhoA/ROCK1通路，促进成骨。因此，TFE至少部分地通过调节细胞骨架中关键基因和蛋白质的表达来促进骨形成。本研究结果为临床应用提供了依据，有助于更好地了解淫羊藿治疗骨缺损的作用机制。
HerbaEpimediiis widely used to promote bone healing, and their active ingredients are total flavonoids ofEpimedium(TFE). Ras homolog gene family member A / Rho‐associated protein kinase (RhoA/Rock), an important pathway regulating the cytoskeleton, has been proven to affect bone formation. However, whether TFE promotes bone healing via this pathway remains unclear. In this study, the therapeutic effects of TFE were estimated using micro‐computed tomography and hematoxylin and eosin staining of pathological sections. F‐actin in osteoblasts was stained to investigate the protective effects of TFE on the cytoskeleton. Its regulatory effects on the RhoA/Rock1 pathway were explored using RT‐qPCR and Western blot analysis. Besides, flow cytometry, alkaline phosphatase and nodule calcification staining were performed to evaluate the effects on osteogenesis. The bone healing in rats was improved, the cytoskeletal damage in osteoblasts was reduced, the RhoA/Rock1 pathway was downregulated, and osteogenesis was enhanced after TFE treatment. Thus, TFE can promote bone formation at least partially by regulating the expression of key genes and proteins in the cytoskeleton. The findings of this study provided evidence for clinical applications and would contribute to a better understanding ofEpimedium’s mechanisms in treating bone defects.