Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures
Effects of the piezo-tolerance of cultured deep-sea eel cells on survival rates, cell proliferation, and cytoskeletal structures
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DOI：
10.1007/s00792-005-0462-3
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发表时间：
2005-12-01
期刊：
影响因子：
2.9
通讯作者：
Aizawa, M
中科院分区：
文献类型：
--
作者：
Koyama, S;Kobayashi, H;Aizawa, M
我们研究了深海鳗(Simenchelys parasiticus；栖息地深度，366-2,630m)细胞、康格尔鳗(Conger Myriaster)细胞和小鼠3T3-L1细胞的压力耐受性。虽然130 Mpa(0.1 Mpa=1bar)静液加压20min后，小鼠3T3-L1和康格尔鳗细胞无一存活，但150 Mpa静压20min后，所有深海鳗鱼细胞均存活。在40兆帕的压力下，20分钟的压力会导致小鼠和鳗鱼细胞中肌动蛋白和微管蛋白细丝的断裂，并导致细胞形状的深刻变化。在深海鳗鱼细胞中，微管和一些肌动蛋白细丝在100 Mpa或更大的静水压力下作用20min后被破坏。康格尔鳗细胞对压力敏感，在10 Mpa条件下不能生长。小鼠3T3-L1细胞在5 Mpa的压力下生长较快，在18 Mpa时停止生长。深海鳗鱼细胞能够在高达25兆帕的压力下生长，在30兆帕时停止生长。深海鳗鱼细胞在20兆帕的压力下需要4h才能完成M期，这大约是大气条件下所需时间的四倍。
We investigated the pressure tolerance of deep-sea eel (Simenchelys parasiticus; habitat depth, 366-2,630 m) cells, conger eel (Conger myriaster) cells, and mouse 3T3-L1 cells. Although there were no living mouse 3T3-L1 and conger eel cells after 130 MPa (0.1 MPa = 1 bar) hydrostatic pressurization for 20 min, all deep-sea eel cells remained alive after being subjected to pressures up to 150 MPa for 20 min. Pressurization at 40 MPa for 20 min induced disruption of actin and tubulin filaments with profound cell-shape changes in the mouse and conger eel cells. In the deep-sea eel cells, microtubules and some actin filaments were disrupted after being subjected to hydrostatic pressure of 100 MPa and greater for 20 min. Conger eel cells were sensitive to pressure and did not grow at 10 MPa. Mouse 3T3-L1 cells grew faster under pressure of 5 MPa than at atmospheric pressure and stopped growing at 18 MPa. Deep-sea eel cells were capable of growth in pressures up to 25 MPa and stopped growing at 30 MPa. Deep-sea eel cells required 4 h at 20 MPa to finish the M phase, which was approximately fourfold the time required under atmospheric conditions.