Interleukin-1 in Lipopolysaccharide Induced Chorioamnionitis in the Fetal Sheep
Interleukin-1 in Lipopolysaccharide Induced Chorioamnionitis in the Fetal Sheep
复制标题
DOI：
10.1177/1933719111404609
复制
发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Kallapur, Suhas G.
中科院分区：
文献类型：
--
作者：
Berry, Clare A.;Nitsos, Ilias;Kallapur, Suhas G.
我们验证了白介素1(IL-1)介导羊膜内脂多糖(LPS)诱导早产羊绒毛膜羊膜炎的假说。单胎配种美利奴母羊在妊娠124+/-1天(n=5~9/组；足月=d 150 d)前1~2天经羊膜腔内注射IL-1α、内毒素或生理盐水(对照组)。羊水中加入重组人IL-1受体拮抗剂(rh IL-1ra)后，于羊水中注入脂多糖或生理盐水，阻断IL-1信号传导。绒毛膜炎症通过组织学、细胞因子信使RNA(MRNA)、蛋白表达和活化炎症细胞的定量来确定。羊膜腔内注射IL-1和内毒素均可诱导绒毛膜羊膜炎。然而，用IL-1ra阻断IL-1并不能减少内毒素引起的羊膜内炎性细胞因子mRNAs、炎症细胞数、髓过氧化物酶或单核细胞趋化蛋白-1表达细胞的增加。结论：IL-1和内毒素均可引起胎羊绒毛膜羊膜炎，但IL-1不是内毒素诱导胎羊绒毛膜羊膜炎的重要介质。
We tested the hypothesis that interleukin 1 (IL-1) mediates intra-amniotic lipopolysaccharide (LPS)-induced chorioamnionitis in pretermfetal sheep. Time-mated Merino ewes with singleton fetuses received IL-1 alpha, LPS, or saline (control) by intra-amniotic injection 1 to 2 days before operative delivery at 124 +/- 1 days gestational age (N = 5-9/group; term =d 150 days). Recombinant human IL-1 receptor antagonist (rhIL-1ra) was given into the amniotic fluid 3 hours before intra-amniotic LPS or saline to block IL-1 signaling. Inflammation in the chorioamnion was determined by histology, cytokine messenger RNA (mRNA), protein expression, and by quantitation of activated inflammatory cells. Intra-amniotic IL-1 and LPS both induced chorioamnionitis. However, IL-1 blockade with IL-1ra did not decrease intra-amniotic LPS-induced increases in pro-inflammatory cytokine mRNAs, numbers of inflammatory cells, myeloperoxidase, or monocyte chemotactic protein-1-expressing cells in the chorioamnion. We conclude that IL-1 and LPS both can cause chorioamnionitis, but IL-1 is not an important mediator of LPS-induced chorioamnionitis in fetal sheep.