VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression
VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression
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DOI：
10.1016/j.atherosclerosis.2011.01.004
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发表时间：
2011-04-01
期刊：
影响因子：
5.3
通讯作者：
Waltenberger, Johannes
中科院分区：
文献类型：
--
作者：
Czepluch, Frauke S.;Olieslagers, Serve;Waltenberger, Johannes
背景：单核细胞募集到血管壁是血管修复、动脉生成和动脉粥样硬化形成的关键初始步骤。两个不同的人类单核细胞亚群可以根据其 CD14/16 表面表达进行分类，即 CD14++CD16-单核细胞 (CD16 mo) 和 CD14+CD16+ 单核细胞 (CD16+mo)。我们研究了两个单核细胞亚群的不同功能特性。方法：通过免疫学选择从人血液中分离CD16 /CD16+mo。我们评估了单核细胞趋化作用、趋化性、粘附和血管内皮生长因子 (VEGF) 受体表达。此外，还研究了活性氧（ROS）的产生以及抗氧化酶的表达。结果：与 CD16 mo 相比，CD16+mo 的趋化作用降低（p
Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets.Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated.Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p