Infection Dynamics of Foot-and-Mouth Disease Virus in Cattle Following Intranasopharyngeal Inoculation or Contact Exposure
Infection Dynamics of Foot-and-Mouth Disease Virus in Cattle Following Intranasopharyngeal Inoculation or Contact Exposure
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DOI：
10.1016/j.jcpa.2016.08.005
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发表时间：
2016-11-01
影响因子：
0.8
通讯作者：
Arzt, J.
中科院分区：
文献类型：
--
作者：
Pacheco, J. M.;Stenfeldt, C.;Arzt, J.
为了建立一种改进的口蹄疫病毒（FMDV）感染牛的实验模型，在标准化的实验条件下，比较了3种不同的实验系统（自然暴露和模拟自然暴露）对FMDV感染牛的影响。通过新型模拟自然鼻咽内（INP）接种系统或通过种内或种间直接接触暴露（牛与牛或猪与牛）的标准化和受控系统，对暴露于FMDV的牛的死前感染动态进行了表征。所有这三种系统都能有效地引起暴露于代表血清型O、A和Asial的三种不同FMDV毒株之一的牛的同步、全身性口蹄疫。与常规使用的针接种系统（上皮内舌接种）相比，自然和模拟自然病毒暴露系统后临床感染的时间有更多的组内变化。然而，本文所述的三种优化的暴露系统具有通过利用原发感染的自然途径接近模拟现场条件的优点，从而促进粘膜宿主防御机制的参与。总体而言，可以得出结论，INP接种和直接接触暴露的标准化系统提供了有效的替代传统（针）接种系统的研究，这是可取的，以模拟自然生物学的口蹄疫病毒感染。爱思唯尔有限公司出版
For the purpose of developing an improved experimental model for studies of foot-and-mouth disease virus (FMDV) infection in cattle, three different experimental systems based on natural or simulated natural virus exposure were compared under standardized experimental conditions. Ante-mortem infection dynamics were characterized in cattle exposed to FMDV through a novel, simulated natural intranasopharyngeal (INP) inoculation system or through standardized and controlled systems of within- or between-species direct contact exposure (cattle-to-cattle or pig-to-cattle). All three systems were efficient in causing synchronous, generalized foot-and-mouth disease in cattle exposed to one of three different strains of FMDV representing serotypes O, A and Asial. There was more within-group variation in the timing of clinical infection following natural and simulated natural virus exposure systems when compared with the conventionally used system of needle inoculation (intraepithelial lingual inoculation). However, the three optimized exposure systems described herein have the advantage of closely simulating field conditions by utilizing natural routes of primary infection, thereby facilitating engagement of mucosal host defence mechanisms. Overall, it is concluded that INP inoculation and standardized systems of direct contact exposure provide effective alternatives to conventional (needle) inoculation systems for studies in which it is desirable to simulate the natural biology of FMDV infection. Published by Elsevier Ltd.