Caffeine as an antioxidant: Inhibition of lipid peroxidation induced by reactive oxygen species
Caffeine as an antioxidant: Inhibition of lipid peroxidation induced by reactive oxygen species
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DOI：
10.1016/0005-2736(96)00040-5
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发表时间：
1996-06-13
影响因子：
3.4
通讯作者：
Kesavan, PC
中科院分区：
文献类型：
--
作者：
Devasagayam, TPA;Kamat, JP;Kesavan, PC
咖啡因（1，3，7-三甲基黄嘌呤），咖啡的成分，已被研究其潜在的抗氧化活性对氧化损伤大鼠肝微粒体。这种损伤是由三种在体内引起膜损伤中至关重要的活性氧物质引起的，即羟基自由基（（OH）-O-），过氧自由基（ROO（.））和单线态氧（O-1（2））。所获得的结果表明，咖啡因是一种有效的脂质过氧化抑制剂，在毫摩尔浓度，对所有三个反应物种。对（OH）-O-引起的过氧化反应有较强的抑制作用，对O-1（2）的抗性中等，对ROO（.）的抗性较低。总的来说，咖啡因的抗氧化能力与已建立的生物抗氧化剂谷胱甘肽相似，且显著高于抗坏血酸。对所观察到的抗氧化作用的可能机制的调查表明，咖啡因对这些活性物质的淬灭可能是负责的可能因素之一。咖啡因与（OH）-O-反应的速率常数。是7.3。10（9）M（-1）s（-1），O-1（2）为2.9。10（7）M（-1）s（-1）。考虑到其潜在的损害，半衰期估计和生物系统中的生成，咖啡因抑制这些反应性物质在膜中诱导的氧化损伤的能力表明咖啡因的一个更积极的属性，其作为咖啡的每日摄入量在大多数人群中可能是相当大的。
Caffeine (1,3,7-trimethyl xanthine), an ingredient of coffee, has been investigated for its potential antioxidant activity against oxidative damage to rat liver microsomes. Such damage was induced by three reactive oxygen species of cardinal importance in causing membrane damage in vivo namely hydroxyl radical ((OH)-O-.), peroxyl radical (ROO(.)) and singlet oxygen (O-1(2)). The results obtained showed that caffeine was an effective inhibitor of lipid peroxidation, at millimolar concentrations, against all the three reactive species. The extent of inhibition was high against peroxidation induced by (OH)-O-., medium against O-1(2) and low against ROO(.). In general, the antioxidant ability of caffeine was similar to that of the established biological antioxidant glutathione and significantly higher than ascorbic acid. Investigations into the possible mechanisms involved in the observed antioxidant effect reveal that the quenching of these reactive species by caffeine may be one of the possible factor responsible. The rate constant of caffeine with (OH)-O-. was 7.3 . 10(9) M(-1) s(-1) and with O-1(2) it was 2.9 . 10(7) M(-1) s(-1). Considering their potential for damage, half-life estimates and generation in biological systems, the ability of caffeine to inhibit oxidative damage induced by these reactive species in membranes suggest one more positive attribute of caffeine, whose daily intake as coffee may be considerable in most populations.