ALLERGENICITY AND ANTIGENICITY OF CHICKEN EGG OVOMUCOID (GAL-D-III) COMPARED WITH OVALBUMIN (GAL-D-I) IN CHILDREN WITH EGG ALLERGY AND IN MICE
ALLERGENICITY AND ANTIGENICITY OF CHICKEN EGG OVOMUCOID (GAL-D-III) COMPARED WITH OVALBUMIN (GAL-D-I) IN CHILDREN WITH EGG ALLERGY AND IN MICE
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DOI：
10.1016/s0091-6749(94)70054-0
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发表时间：
1994-06-01
影响因子：
14.2
通讯作者：
SAMPSON, HA
中科院分区：
文献类型：
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作者：
BERNHISELBROADBENT, J;DINTZIS, HM;SAMPSON, HA
当试图产生针对母鸡卵白蛋白的小鼠单克隆抗体时，注射市售纯化的卵白蛋白产生单克隆抗体，当对市售纯化的卵白蛋白（Gal d I）或卵粘液（Gal d III）进行检测时，该单克隆抗体似乎对两者都具有特异性。利用高效液相色谱法（HPLC）对卵清蛋白和类卵粘蛋白进行再纯化，发现由商业纯化的卵清蛋白产生的单克隆抗体仅对类卵粘蛋白具有特异性。为了澄清这一现象，小鼠被连续注射了市售纯化的卵清蛋白或hplc再纯化的卵清蛋白。结果发现，对商业纯化卵清蛋白的大部分抗体反应是针对次要的(
When attempting to generate mouse monoclonal antibodies to hen's egg ovalbumin, injection of commercially purified ovalbumin resulted in monoclonal antibodies, which when assayed against commercially purified ovalbumin (Gal d I) or ovomucoid (Gal d III), apppeared to be specific to both. With the use of high-performance liquid chromatography (HPLC)-repurified ovalbumin and ovomucoid in assay procedures, monoclonal antibodies generated by commercially purified ovalbumin were found to be specific for ovomucoid only. To clarify, this phenomenon, mice were serially injected with commercially purified ovalbumin or HPLC-repurified ovalbumin. It was found that most of the antibody response to commercially purified ovalbumin was directed against the minor (