Gene expression profiles in human lymphocytes irradiated in vitro with low doses of gamma rays
Gene expression profiles in human lymphocytes irradiated in vitro with low doses of gamma rays
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DOI：
10.1667/rr0487.1
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发表时间：
2007-12-01
影响因子：
3.4
通讯作者：
Sakamoto-Hojo, Elza T.
中科院分区：
文献类型：
--
作者：
Fachin, Ana L.;Mello, Stephano S.;Sakamoto-Hojo, Elza T.
低剂量辐射反应的分子机制尚不清楚，特别是在正常淋巴细胞中，尽管有证据表明细胞反应可能具有特定的变化。我们的目的是用DNA芯片分析人淋巴细胞在体外(10、25和50cGy射线)照射后的基因表达谱。并对不同的辐射剂量进行了细胞遗传学分析。G(0)淋巴细胞照射诱导增殖48h后，采集RNA样本进行基因表达分析。ANOVA被应用于四个实验中获得的数据，四个健康捐赠者的数据随后进行了SAM分析和等级聚类。当照射剂量为10、25和50cGy时，FDR=10cGy(总像差)和GT=50cGy(双着丝粒/环)的数目显著增加。因此，低到中等剂量的辐射会导致转录谱在定性和/或定量上的差异和相似，反映了DNA损伤的类型和程度。与调控基因相关的主要生物学过程是代谢、应激反应/DNA修复、细胞生长/分化和转录调控。这些结果表明，人类面临着遗传不稳定和获得性疾病的潜在风险。(C)2007年，由辐射研究学会提供。
The molecular mechanisms underlying responses to low radiation doses are still unknown, especially in normal lymphocytes, despite the evidence suggesting specific changes that may characterize cellular responses. Our purpose was to analyze gene expression profiles by DNA microarrays in human lymphocytes after in vitro irradiation (10, 25 and 50 cGy) with gamma rays. A cytogenetic analysis was also carried out for different radiation doses. G(0) lymphocytes were irradiated and induced to proliferate for 48 h; then RNA samples were collected for gene expression analysis. ANOVA was applied to data obtained in four experiments with four healthy donors, followed by SAM analysis and hierarchical clustering. For 10, 25 and 50 cGy, the numbers of significantly (FDR = 10 cGy (total aberrations) and >= 50 cGy (dicentrics/rings). Therefore, low to moderate radiation doses induced qualitative and/or quantitative differences and similarities in transcript profiles, reflecting the type and extent of DNA lesions. The main biological processes associated with modulated genes were metabolism, stress response/DNA repair,cell growth/differentiation, and transcription regulation. The results indicate a potential risk to humans regarding the development of genetic instability and acquired diseases. (c) 2007by Radiation Research Societ.