Evaluation of adhesion, proliferation, and functional differentiation of dermal fibroblasts on glycosaminoglycan-coated polysulfone membranes.
Evaluation of adhesion, proliferation, and functional differentiation of dermal fibroblasts on glycosaminoglycan-coated polysulfone membranes.
复制标题
DOI：
10.1089/ten.tea.2007.0057
复制
发表时间：
2008-09
影响因子：
--
通讯作者：
J. Attia;F. Legendre;Q. Nguyen;C. Baugé;K. Boumediene;J. Pujol
中科院分区：
文献类型：
--
作者：
J. Attia;F. Legendre;Q. Nguyen;C. Baugé;K. Boumediene;J. Pujol
可以假设，伤口愈合将受益于涂有细胞外基质大分子的聚合物膜。在这里，我们描述了真皮成纤维细胞在聚砜（PSU）膜和覆盖有壳聚糖（Ch）、硫酸软骨素（CS）或透明质酸的PSU膜上的行为，使用聚（丙烯腈-共-甲代烯丙基磺酸盐）（AN69）进行额外的中间离子电荷修饰，允许结合 多糖大分子。研究了细胞粘附、增殖、细胞信号传导和胶原蛋白基因表达。 Ch 和 CS 被发现无法支持细胞粘附和增殖。相比之下，PSU 和透明质酸涂层的 PSU 膜似乎都是培养成纤维细胞并支持其基质合成能力的合适材料。此外，除了 I 型胶原蛋白外，它们还诱导 III 型胶原蛋白表达，这表明它们促进了类似胎儿的环境，可能有利于伤口愈合。
It may be hypothesized that wound healing will benefit from polymer membranes coated with extracellular matrix macromolecules. Here we describe the behavior of dermal fibroblasts on polysulfone (PSU) membranes, and PSU membranes covered with chitosan (Ch), chondroitin sulfate (CS), or hyaluronan, using an additional intermediary ionic charge modification with poly-(acrylonitrile-co-methallyl sulfonate) (AN69), which allows binding of the polysaccharidic macromolecules. Cell adhesion, proliferation, cell signaling, and collagen gene expression were investigated. Ch and CS were found unable to support cell adhesion and proliferation. In contrast, both PSU and hyaluronic acid-coated PSU membranes appeared as suitable materials to culture fibroblasts and support their matrix synthesis capacity. Moreover, they induce type III collagen expression in addition to type I, suggesting that they promote a fetal-like environment that could be beneficial for wound healing.