DIFFERENCES IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) ANTI-P24 REACTIVITIES IN SERUM OF HIV-1-INFECTED AND UNINFECTED SUBJECTS - ANALYSIS OF INDETERMINATE WESTERN-BLOT REACTIONS
DIFFERENCES IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) ANTI-P24 REACTIVITIES IN SERUM OF HIV-1-INFECTED AND UNINFECTED SUBJECTS - ANALYSIS OF INDETERMINATE WESTERN-BLOT REACTIONS
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DOI：
10.1093/infdis/163.2.247
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发表时间：
1991-02-01
影响因子：
6.4
通讯作者：
ARMSTRONG, D
中科院分区：
文献类型：
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作者：
POVOLOTSKY, J;GOLD, JWM;ARMSTRONG, D
本文用免疫印迹法检测了42例HIV-1感染者和36例非感染者血清中抗p24蛋白的反应。 HIV-1感染者的抗p24反应被HIV-1重组p24抗原完全阻断，而未感染者的不确定模式的反应被部分抑制或完全抑制。 与感染个体相反，未感染个体的抗p24反应性不能通过HIV-1 p24抗体的抑制ELISA检测到。 这些结果表明，一个实用的方法来区分抗p24的反应，从未感染的患者与不确定的蛋白质印迹反应的HIV-1感染患者的血清。
Anti-p24 reactions in sera from 42 human immunodeficiency virus type 1 (HIV-1)-infected and 36 uninfected people with indeterminate Western blot antibody tests for HIV-1 were studied. Anti-p24 reactivity in HIV-1-infected people was completely blocked by HIV-1 recombinant p24 antigen, whereas the reaction in uninfected individuals with indeterminate patterns was inhibited partly or not at all. In contrast to infected individuals, anti-p24 reactivity from uninfected individuals could not be detected by an inhibition ELISA for HIV-1 p24 antibody. These results suggest a practical way to distinguish anti-p24 reactivity in sera from patients with HIV-1 infection from that seen in uninfected patients with indeterminate Western blot reactions.